Effects of Chlorhexidine mouthwash on the oral microbiome.
Adult
Biomarkers
Blood Pressure
/ drug effects
Chlorhexidine
/ pharmacology
Cross-Over Studies
Female
Glucose
/ analysis
Humans
Hydrogen-Ion Concentration
Lactates
/ analysis
Male
Microbiota
/ drug effects
Mouth
/ microbiology
Mouthwashes
/ pharmacology
Nitrates
/ analysis
Nitrites
/ analysis
Saliva
/ chemistry
Single-Blind Method
Species Specificity
Young Adult
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
24 03 2020
24 03 2020
Historique:
received:
02
10
2019
accepted:
26
02
2020
entrez:
27
3
2020
pubmed:
27
3
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Following a single blind, cross-over and non-randomized design we investigated the effect of 7-day use of chlorhexidine (CHX) mouthwash on the salivary microbiome as well as several saliva and plasma biomarkers in 36 healthy individuals. They rinsed their mouth (for 1 min) twice a day for seven days with a placebo mouthwash and then repeated this protocol with CHX mouthwash for a further seven days. Saliva and blood samples were taken at the end of each treatment to analyse the abundance and diversity of oral bacteria, and pH, lactate, glucose, nitrate and nitrite concentrations. CHX significantly increased the abundance of Firmicutes and Proteobacteria, and reduced the content of Bacteroidetes, TM7, SR1 and Fusobacteria. This shift was associated with a significant decrease in saliva pH and buffering capacity, accompanied by an increase in saliva lactate and glucose levels. Lower saliva and plasma nitrite concentrations were found after using CHX, followed by a trend of increased systolic blood pressure. Overall, this study demonstrates that mouthwash containing CHX is associated with a major shift in the salivary microbiome, leading to more acidic conditions and lower nitrite availability in healthy individuals.
Identifiants
pubmed: 32210245
doi: 10.1038/s41598-020-61912-4
pii: 10.1038/s41598-020-61912-4
pmc: PMC7093448
doi:
Substances chimiques
Biomarkers
0
Lactates
0
Mouthwashes
0
Nitrates
0
Nitrites
0
Glucose
IY9XDZ35W2
Chlorhexidine
R4KO0DY52L
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5254Références
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