The exogenous application of AtPGLR, an endo-polygalacturonase, triggers pollen tube burst and repair.
Amaryllis belladonna
Arabidopsis thaliana
elongation
oligogalacturonides
pectins
pollen tube
polygalacturonase
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
25
07
2019
revised:
14
02
2020
accepted:
12
03
2020
pubmed:
28
3
2020
medline:
6
3
2021
entrez:
28
3
2020
Statut:
ppublish
Résumé
Plant cell wall remodeling plays a key role in the control of cell elongation and differentiation. In particular, fine-tuning of the degree of methylesterification of pectins was previously reported to control developmental processes as diverse as pollen germination, pollen tube elongation, emergence of primordia or elongation of dark-grown hypocotyls. However, how pectin degradation can modulate plant development has remained elusive. Here we report the characterization of a polygalacturonase (PG), AtPGLR, the gene for which is highly expressed at the onset of lateral root emergence in Arabidopsis. Due to gene compensation mechanisms, mutant approaches failed to determine the involvement of AtPGLR in plant growth. To overcome this issue, AtPGLR has been expressed heterologously in the yeast Pichia pastoris and biochemically characterized. We showed that AtPGLR is an endo-PG that preferentially releases non-methylesterified oligogalacturonides with a short degree of polymerization (< 8) at acidic pH. The application of the purified recombinant protein on Amaryllis pollen tubes, an excellent model for studying cell wall remodeling at acidic pH, induced abnormal pollen tubes or cytoplasmic leakage in the subapical dome of the pollen tube tip, where non-methylesterified pectin epitopes are detected. Those leaks could either be repaired by new β-glucan deposits (mostly callose) in the cell wall or promoted dramatic burst of the pollen tube. Our work presents the full biochemical characterization of an Arabidopsis PG and highlights the importance of pectin integrity in pollen tube elongation.
Substances chimiques
Arabidopsis Proteins
0
Polygalacturonase
EC 3.2.1.15
Banques de données
RefSeq
['At5g14650', 'At5g25760', 'At5g46630', 'At1g27450', 'At1g05660', 'At2g43880', 'At2g43890', 'At3g26610', 'At3g59850', 'At3g06770', 'At3g16850', 'At3g62110']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
617-633Informations de copyright
© 2020 Society for Experimental Biology and John Wiley & Sons Ltd.
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