Needle in a haystack: Antibacterial activity-guided fractionation of a potato wound tissue extract.
Antibacterial
Erwinia carotovora
LC-MS
Potato
Solanum tuberosum
TOF-MS
Wound periderm
Journal
Bioorganic & medicinal chemistry
ISSN: 1464-3391
Titre abrégé: Bioorg Med Chem
Pays: England
ID NLM: 9413298
Informations de publication
Date de publication:
01 05 2020
01 05 2020
Historique:
received:
06
08
2019
revised:
10
02
2020
accepted:
06
03
2020
pubmed:
29
3
2020
medline:
29
5
2021
entrez:
29
3
2020
Statut:
ppublish
Résumé
Erwinia carotovora is a major cause of potato tuber infection, which results in disastrous failures of this important food crop. There is currently no effective antibiotic treatment against E. carotovora. Recently we reported antibacterial assays of wound tissue extracts from four potato cultivars that exhibit a gradient of russeting character, finding the highest potency against this pathogen for a polar extract from the tissue formed immediately after wounding by an Atlantic cultivar. In the current investigation, antibacterial activity-guided fractions of this extract were analyzed by liquid chromatography-mass spectrometry (LC-MS) utilizing a quadrupole-time-of-flight (QTOF) mass spectrometer. The most active chemical compounds identified against E. carotovora were: 6-O-nonyl glucitol, Lyratol C, n-[2-(4-Hydroxyphenyl)] ethyldecanamide, α-chaconine and α-solanine. Interactions among the three compounds, ferulic acid, feruloyl putrescine, and α-chaconine, representing metabolite classes upregulated during initial stages of wound healing, were also evaluated, offering possible explanations for the burst in antibacterial activity after tuber wounding and a chemical rationale for the temporal resistance phenomenon.
Identifiants
pubmed: 32216988
pii: S0968-0896(20)30238-8
doi: 10.1016/j.bmc.2020.115428
pmc: PMC7237834
mid: NIHMS1575002
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Tissue Extracts
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
115428Subventions
Organisme : NIMHD NIH HHS
ID : G12 MD007603
Pays : United States
Organisme : NCRR NIH HHS
ID : G12 RR003060
Pays : United States
Organisme : NIGMS NIH HHS
ID : T34 GM007639
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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