Evaluating Intrahepatic and Intraperitoneal Sodium Pentobarbital or Ethanol for Mouse Euthanasia.
Anesthetics, Inhalation
/ administration & dosage
Animals
Drug Administration Routes
Ethanol
/ administration & dosage
Euthanasia, Animal
/ methods
Female
Guidelines as Topic
Injections, Intraperitoneal
Isoflurane
/ administration & dosage
Male
Mice
Pentobarbital
/ administration & dosage
Prospective Studies
Random Allocation
Specific Pathogen-Free Organisms
Journal
Journal of the American Association for Laboratory Animal Science : JAALAS
ISSN: 2769-6677
Titre abrégé: J Am Assoc Lab Anim Sci
Pays: United States
ID NLM: 101269489
Informations de publication
Date de publication:
01 05 2020
01 05 2020
Historique:
pubmed:
30
3
2020
medline:
28
1
2021
entrez:
30
3
2020
Statut:
ppublish
Résumé
Intraperitoneal (IP) injection of sodium pentobarbital (PB) is an accepted method of euthanasia for mice. However, this method has important drawbacks, including the potential for pain or misinjection. The objective of this prospective, randomized, blinded study was to determine whether intrahepatic (IH) injection of PB is more effective than IP delivery for mouse euthanasia. Secondary objectives were to: 1) determine whether IP ethanol (ET) is a suitable alternative to PB and 2) study the effect of isoflurane anesthesia on euthanasia with either PB or ET. Eighty adult CD1 mice were randomly assigned to 6 different treatment groups, were euthanized by using IP or IH injections of either PB or ET, and were either anesthetized or conscious before injection. Variables of interest were: 1) misinjection rates (based on necropsy evaluation), 2) time from injection to apnea and 3) time to cessation of heartbeat (CHB). The misinjection rate for IH injections was 93% (28/30). Two successful IH injections resulted in death within 4 s, but this method cannot be recommended due to the possibility for intrathoracic injection (
Identifiants
pubmed: 32220260
doi: 10.30802/AALAS-JAALAS-19-000097
pmc: PMC7210731
doi:
Substances chimiques
Anesthetics, Inhalation
0
Ethanol
3K9958V90M
Isoflurane
CYS9AKD70P
Pentobarbital
I4744080IR
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
264-268Références
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