Bisphenol A analogues (BPS and BPF) present a greater obesogenic capacity in 3T3-L1 cell line.
3T3-L1 Cells
Animals
Benzhydryl Compounds
/ toxicity
CCAAT-Enhancer-Binding Protein-alpha
/ metabolism
Cell Differentiation
/ drug effects
Endocrine Disruptors
/ toxicity
Fatty Acid-Binding Proteins
/ metabolism
Lipid Metabolism
/ drug effects
Mice
Obesity
/ chemically induced
PPAR gamma
/ metabolism
Phenols
/ toxicity
Sulfones
/ toxicity
Up-Regulation
/ drug effects
Adipogenesis
Analogues
Bisphenol A (BPA)
Cell viability
Endocrine potential activity
Journal
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
ISSN: 1873-6351
Titre abrégé: Food Chem Toxicol
Pays: England
ID NLM: 8207483
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
12
02
2020
revised:
09
03
2020
accepted:
19
03
2020
pubmed:
30
3
2020
medline:
20
2
2021
entrez:
30
3
2020
Statut:
ppublish
Résumé
This study was aimed at comparing the toxicity effects on cell viability and the obesogenic activity of Bisphenol A (BPA) and its analogues, Bisphenol S (BPS) and Bisphenol F (BPF), by in vitro assays with a preadipocytic 3T3-L1 cell line. To compare the toxic potential and select the concentrations of each chemical not showing a decrease in cell viability, MTT assay was performed. The cell phenotype was determined in differentiated 3T3-L1 adipocytes by red oil O staining. To determine the expression levels of the different adipogenic proteins the Western Blot test was performed. The results from MTT assay showed a greater toxic effect of BPA - at equal and even lower concentrations-than its analogues. However, BPS followed by BPF showed a greater neutral lipid storage capacity than BPA, which was reflected in the increase of the protein expression of CCAAT/enhancer binding protein α (C/EBPα), peroxisome proliferator-activated receptor gamma γ (PPARγ) and acid-binding protein 4 (FABP4). In summary, these BPA analogues -especially BPS- present a greater endocrine potential activity than BPA.
Identifiants
pubmed: 32220626
pii: S0278-6915(20)30186-1
doi: 10.1016/j.fct.2020.111298
pii:
doi:
Substances chimiques
Benzhydryl Compounds
0
CCAAT-Enhancer-Binding Protein-alpha
0
Endocrine Disruptors
0
Fabp4 protein, mouse
0
Fatty Acid-Binding Proteins
0
PPAR gamma
0
Phenols
0
Sulfones
0
bisphenol F
0
bis(4-hydroxyphenyl)sulfone
80-09-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111298Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.