Isolation of Leishmania Promastigote Flagella.
Cell fractionation
Flagella
Kinetoplastids
Leishmania
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
30
3
2020
pubmed:
30
3
2020
medline:
2
2
2021
Statut:
ppublish
Résumé
Eukaryotic flagella are conserved multifunctional organelles with roles in motility, intercellular interactions, and signal transduction. Leishmania possess a single flagellum at all stages of their life cycle. Flagella of promastigote forms in the fly are long and motile, with a canonical 9 + 2 microtubule axoneme and an extra-axonemal paraflagellar rod (PFR). This protocol describes a simple method for the isolation of Leishmania mexicana promastigote flagella, optimized to yield intact flagella that retain both the cytoskeletal elements (9 + 2 axoneme and PFR) and the surrounding membrane. The isolated flagella and deflagellated cell bodies are suitable for analysis by electron microscopy, protein mass spectrometry, and lipidomics.
Identifiants
pubmed: 32221938
doi: 10.1007/978-1-0716-0294-2_29
doi:
Substances chimiques
Protozoan Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
485-495Subventions
Organisme : Wellcome Trust
ID : 211075/Z/18/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : 15/16_MSD_836338
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 211,075/Z/18/Z
Pays : United Kingdom