Molecular alterations in non-functioning pituitary adenomas.
Adenoma
/ genetics
Biomarkers, Tumor
/ genetics
Calcium Channels, L-Type
/ genetics
Computational Biology
Datasets as Topic
Gene Expression Regulation, Neoplastic
Homeodomain Proteins
/ genetics
Humans
Kruppel-Like Transcription Factors
/ genetics
Oligonucleotide Array Sequence Analysis
Pituitary Gland
/ pathology
Pituitary Neoplasms
/ genetics
Receptors, CXCR4
/ genetics
Transcription Factors
/ genetics
Homeobox Protein PITX2
Non-functioning pituitary adenomas
gonadotropinomas
molecular markers
null cell
Journal
Cancer biomarkers : section A of Disease markers
ISSN: 1875-8592
Titre abrégé: Cancer Biomark
Pays: Netherlands
ID NLM: 101256509
Informations de publication
Date de publication:
2020
2020
Historique:
pubmed:
1
4
2020
medline:
20
1
2021
entrez:
1
4
2020
Statut:
ppublish
Résumé
Clinically non-functioning Pituitary Adenomas (NFPA) are among the most common neoplasms of the sellar region. They usually present with compressive symptoms such as headache and visual field defects and not infrequently, are found incidentally. NFPA are classified as gonadotropinomas, null cell adenomas, according to their immunohistochemical phenotype. The molecular alterations responsible for the development of these lesions are incompletely understood, and there is scarce information regarding the molecular alterations and markers. We carried out an in-silico analysis aimed at identifying the molecular alterations in NFPA and to discover new molecular markers. Twenty-three microarray libraries were analyzed. Fourteen correspond to NFPA and 9 to control tissue gland. They were analyzed using Partek Genomic Suite to identify differentially expressed genes and WebGestalt and Metascape to understand the meaning behind the gene lists. Pituitary adenomas showed a markedly different transcriptome compared to the non-tumoral gland, regardless of their putative immunophenotype. Genes related to calcium metabolism such as CACNA2D4, immune-related CXCR4, and stem cell-related KLF8 and PITX2 were altered. Differentially expressed calcium metabolism and immune-related genes in NFPA represent attractive molecular markers and potential therapeutic targets.
Sections du résumé
BACKGROUND
BACKGROUND
Clinically non-functioning Pituitary Adenomas (NFPA) are among the most common neoplasms of the sellar region. They usually present with compressive symptoms such as headache and visual field defects and not infrequently, are found incidentally. NFPA are classified as gonadotropinomas, null cell adenomas, according to their immunohistochemical phenotype. The molecular alterations responsible for the development of these lesions are incompletely understood, and there is scarce information regarding the molecular alterations and markers.
OBJECTIVE
OBJECTIVE
We carried out an in-silico analysis aimed at identifying the molecular alterations in NFPA and to discover new molecular markers.
METHODS
METHODS
Twenty-three microarray libraries were analyzed. Fourteen correspond to NFPA and 9 to control tissue gland. They were analyzed using Partek Genomic Suite to identify differentially expressed genes and WebGestalt and Metascape to understand the meaning behind the gene lists.
RESULTS
RESULTS
Pituitary adenomas showed a markedly different transcriptome compared to the non-tumoral gland, regardless of their putative immunophenotype. Genes related to calcium metabolism such as CACNA2D4, immune-related CXCR4, and stem cell-related KLF8 and PITX2 were altered.
CONCLUSIONS
CONCLUSIONS
Differentially expressed calcium metabolism and immune-related genes in NFPA represent attractive molecular markers and potential therapeutic targets.
Identifiants
pubmed: 32224525
pii: CBM191121
doi: 10.3233/CBM-191121
doi:
Substances chimiques
Biomarkers, Tumor
0
CACNA2D4 protein, human
0
CXCR4 protein, human
0
Calcium Channels, L-Type
0
Homeodomain Proteins
0
KLF8 protein, human
0
Kruppel-Like Transcription Factors
0
Receptors, CXCR4
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM