Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children.
Adolescent
Anti-Bacterial Agents
/ pharmacology
Child
Child Health Services
Child, Preschool
Community-Acquired Infections
/ drug therapy
Cross-Sectional Studies
Electrophoresis, Gel, Pulsed-Field
Female
Humans
Infant
Infant, Newborn
Male
Methicillin-Resistant Staphylococcus aureus
/ genetics
Multilocus Sequence Typing
Paraguay
/ epidemiology
Staphylococcal Infections
/ drug therapy
Virulence Factors
MRSA
Methicillin-resistant Staphylococcus aureus
children
community-associated
Journal
Journal of infection in developing countries
ISSN: 1972-2680
Titre abrégé: J Infect Dev Ctries
Pays: Italy
ID NLM: 101305410
Informations de publication
Date de publication:
31 03 2020
31 03 2020
Historique:
received:
14
10
2019
accepted:
24
02
2020
entrez:
3
4
2020
pubmed:
3
4
2020
medline:
5
1
2021
Statut:
epublish
Résumé
Staphylococcus aureus is considered one of the most important human pathogens, and its levels of resistance to methicillin have increased even in strains isolated from people without nosocomial risk factors. Molecular analysis is essential for understanding the patterns of dissemination. The objective of this study was to identify community-acquired methicillin-resistant S. aureus (CA-MRSA) clones that infected Paraguayan children patients in two periods of time. An observational, descriptive study was designed to determine the genetic variability of 115 isolates of CA-MRSA recovered from children who attended four reference centers in Paraguay between 2009-2010 and 2012-2013. The combined use of Pulsed Field Gel Electrophoresis (PFGE), Multi-Locus Sequencing Typing, Multi-Locus Variable Analysis (MLVA) and Spa typing techniques allowed the identification of two dominant clones: ST30-IV-t019 (77%) and ST5-IV-t311 (10%), and the establishment of the former as the leading cause of CA-MRSA infections in children during the study period. This is the first study that provides epidemiological information as well as microbiological and molecular characteristics of CA-MRSA isolates recovered from children from Asunción and the Central Department of Paraguay.
Substances chimiques
Anti-Bacterial Agents
0
Virulence Factors
0
Types de publication
Journal Article
Multicenter Study
Observational Study
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
290-297Informations de copyright
Copyright (c) 2020 Fatima Rodriguez, Claudia Salinas, Silvina Fernandez, Sol Haim, Marta Mollerach, Wilma Basualdo, Hector Castro, Beatriz Quinonez, Rocio Arguello, Monica Rodriguez, Lorena Grau, Carmen Espínola, Gladys Velazquez, Gloria Samudio, Gloria Gomez, Ana Campuzano, Juana Ortellado, Patricia Almada, Rosa Guillen.
Déclaration de conflit d'intérêts
No Conflict of Interest is declared