Anti-inflammatory Effect of Curcuma longa and Allium hookeri Co-treatment via NF-κB and COX-2 Pathways.
Allium
Animals
Anti-Inflammatory Agents
/ pharmacology
Cell Proliferation
/ drug effects
Curcuma
Cyclooxygenase 2
/ metabolism
Inflammation
/ metabolism
Inflammation Mediators
/ metabolism
Male
Mice
Mice, Inbred ICR
NF-kappa B
/ metabolism
Plant Extracts
/ pharmacology
RAW 264.7 Cells
Skin
/ drug effects
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
31 03 2020
31 03 2020
Historique:
received:
17
12
2019
accepted:
19
03
2020
entrez:
3
4
2020
pubmed:
3
4
2020
medline:
2
12
2020
Statut:
epublish
Résumé
Although inflammation is a host defense mechanism, chronic inflammation mediates several diseases, including cancer, allergy, asthma, and autoimmune diseases, and reportedly, it is associated with a 60% mortality rate. There are several reports on the anti-inflammatory effects of Curcuma longa and Allium hookeri. However, although they can be used as culinary materials and have biological effects, they are not effective anti-inflammatory agents. In this study, we evaluated the synergic effect of C. longa and A. hookeri in order to confirm the possibility of a new anti-inflammatory agent. Based on cell viability and cytokine analyses, the appropriate ratio of C. longa and A. hookeri was confirmed using an air pouch animal model. Then, the anti-inflammatory effect of C. longa and A. hookeri co-treatment was evaluated by measuring the immune cell count and cytokines in the exudate and by comparing the morphological changes and cytokines in inflamed skin samples. Additionally, we evaluated the NF-κB/COX-2 pathway and iNOS levels. The active constituents detected in C. longa were demethoxycurcumin and bisdemethoxycurcumin, and that detected in A. hookeri was methylsulfonylmethane. An in vitro assessment determined the appropriate drug ratio as 3:7. In a carrageenan-induced inflammatory model, co-treatment effectively suppressed inflammatory cytokines, including IFN-γ, IL-1β, IL-6, IL-13, and IL-17, and recovered inflammation-related morphological changes in the skin. The anti-inflammatory effect of the co-treatment was mediated through the NF-κB/COX-2 pathway and iNOS inhibition. We concluded that co-treatment with C. longa and A. hookeri synergistically inhibited inflammation via the NF-κB/COX-2/iNOS pathway.
Identifiants
pubmed: 32235914
doi: 10.1038/s41598-020-62749-7
pii: 10.1038/s41598-020-62749-7
pmc: PMC7109078
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Inflammation Mediators
0
NF-kappa B
0
Plant Extracts
0
Cyclooxygenase 2
EC 1.14.99.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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