Rapid Expansion of Highly Functional Antigen-Specific T Cells from Patients with Melanoma by Nanoscale Artificial Antigen-Presenting Cells.
Antigen Presentation
/ immunology
Antigen-Presenting Cells
/ immunology
Antigens, Neoplasm
/ immunology
Biomarkers
Cell Line, Tumor
Cytokines
/ metabolism
Dendritic Cells
/ immunology
Humans
Immunophenotyping
Lymphocyte Activation
/ immunology
Lymphocyte Count
Melanoma
/ immunology
Models, Biological
T-Cell Antigen Receptor Specificity
T-Lymphocyte Subsets
/ immunology
T-Lymphocytes
/ immunology
Journal
Clinical cancer research : an official journal of the American Association for Cancer Research
ISSN: 1557-3265
Titre abrégé: Clin Cancer Res
Pays: United States
ID NLM: 9502500
Informations de publication
Date de publication:
01 07 2020
01 07 2020
Historique:
received:
23
10
2019
revised:
13
02
2020
accepted:
30
03
2020
pubmed:
4
4
2020
medline:
15
9
2021
entrez:
4
4
2020
Statut:
ppublish
Résumé
Generation of antigen-specific T cells from patients with cancer employs large numbers of peripheral blood cells and/or tumor-infiltrating cells to generate antigen-presenting and effector cells commonly requiring multiple rounds of restimulation Nano-aAPC-expressing HLA-A*0201 molecules and costimulatory anti-CD28 antibody and HLA-A*0201 molecules loaded with MART-1 or gp100 class I-restricted peptides were used to stimulate CD8 T cells purified from the peripheral blood of treatment-naïve or PD-1 antibody-treated patients with stage IV melanoma. Expanded cells were restimulated with fresh peptide-pulsed nano-aAPC at day 7. Phenotype analysis and functional assays including cytokine release, cytolysis, and measurement of avidity were conducted. MART-1-specific CD8 T cells rapidly expanded up to 1,000-fold by day 14 after exposure to peptide-pulsed nano-aAPC. Expanded T cells had a predominantly stem cell memory CD45RA Peptide-pulsed nano-aAPC rapidly expanded polyfunctional antigen-specific CD8 T cells with high avidity, potent lytic function, and a stem cell memory phenotype from patients with melanoma.
Identifiants
pubmed: 32241816
pii: 1078-0432.CCR-19-3487
doi: 10.1158/1078-0432.CCR-19-3487
pmc: PMC7334099
mid: NIHMS1582183
doi:
Substances chimiques
Antigens, Neoplasm
0
Biomarkers
0
Cytokines
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3384-3396Subventions
Organisme : NCI NIH HHS
ID : R33 CA229042
Pays : United States
Organisme : NCI NIH HHS
ID : R00 CA230201
Pays : United States
Organisme : NIBIB NIH HHS
ID : P41 EB028239
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA175732
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007057
Pays : United States
Organisme : NCI NIH HHS
ID : K99 CA230201
Pays : United States
Informations de copyright
©2020 American Association for Cancer Research.
Références
Nat Biotechnol. 2018 Feb;36(2):160-169
pubmed: 29334370
J Exp Med. 2016 Jun 27;213(7):1133-9
pubmed: 27242164
J Immunother. 2003 Sep-Oct;26(5):440-50
pubmed: 12973033
Cytotherapy. 2009;11(7):912-22
pubmed: 19903103
N Engl J Med. 2008 Jun 19;358(25):2698-703
pubmed: 18565862
Small. 2015 Apr;11(13):1519-25
pubmed: 25641795
Nat Med. 2016 Apr;22(4):433-8
pubmed: 26901407
Science. 2015 May 15;348(6236):803-8
pubmed: 25837513
Front Immunol. 2018 Nov 28;9:2798
pubmed: 30546367
Mol Ther Methods Clin Dev. 2014 Mar 05;1:14001
pubmed: 26015949
Clin Exp Immunol. 2011 Jan;163(1):1-10
pubmed: 20964638
Immunol Rev. 2014 Jan;257(1):250-63
pubmed: 24329802
Int Immunol. 2010 Nov;22(11):863-73
pubmed: 21059769
J Clin Invest. 2016 Sep 1;126(9):3363-76
pubmed: 27482888
Cancer Immunol Immunother. 2012 Feb;61(2):289-293
pubmed: 22237887
Nat Med. 2001 Oct;7(10):1159-62
pubmed: 11590442
Haematologica. 2008 Oct;93(10):1523-34
pubmed: 18728033
ACS Nano. 2015 Jul 28;9(7):6861-71
pubmed: 26171764
Cancer Res. 2008 Apr 15;68(8):3010-8
pubmed: 18413771
Oncoimmunology. 2012 Mar 1;1(2):172-178
pubmed: 22720238
Nat Med. 2017 Jan 6;23(1):18-27
pubmed: 28060797
N Engl J Med. 2015 Jan 22;372(4):320-30
pubmed: 25399552
J Immunol. 2017 May 1;198(9):3507-3514
pubmed: 28341670
Science. 2014 Dec 19;346(6216):1536-40
pubmed: 25525252
Biomaterials. 2017 Feb;118:16-26
pubmed: 27940380
Mol Immunol. 2009 Feb;46(5):893-901
pubmed: 19041139
Nanomedicine. 2014 Jan;10(1):119-29
pubmed: 23891987
J Transl Med. 2010 Oct 26;8:104
pubmed: 20977748
Clin Cancer Res. 2011 Aug 15;17(16):5343-52
pubmed: 21737507
J Immunol. 2003 Sep 1;171(5):2251-61
pubmed: 12928369
Clin Cancer Res. 2006 May 15;12(10):2967-75
pubmed: 16707591
Curr Opin Immunol. 2017 Apr;45:43-51
pubmed: 28192720
Nat Med. 2003 May;9(5):619-24
pubmed: 12704385
Sci Transl Med. 2013 Feb 27;5(174):174ra27
pubmed: 23447018
Nat Nanotechnol. 2014 Aug;9(8):639-47
pubmed: 25086604
J Immunol Methods. 2005 Jul;302(1-2):145-55
pubmed: 15992809
Clin Cancer Res. 2010 May 1;16(9):2646-55
pubmed: 20406835
Curr Opin Immunol. 2009 Apr;21(2):233-40
pubmed: 19304471
N Engl J Med. 2015 Jul 2;373(1):23-34
pubmed: 26027431
Semin Cancer Biol. 2012 Aug;22(4):298-306
pubmed: 22414911
Cancer Immunol Immunother. 2008 Feb;57(2):175-83
pubmed: 17657490
J Immunol. 1999 Feb 15;162(4):2227-34
pubmed: 9973498
J Immunol. 2010 Jan 1;184(1):452-65
pubmed: 19949105
Adv Mater. 2019 Jun;31(23):e1807359
pubmed: 30968468
J Immunother. 2009 Feb-Mar;32(2):169-80
pubmed: 19238016
Nat Protoc. 2014 Apr;9(4):950-66
pubmed: 24675735
Lancet Oncol. 2016 Jul;17(7):943-955
pubmed: 27269740
Clin Cancer Res. 2007 Mar 15;13(6):1857-67
pubmed: 17363542
J Biol Chem. 2011 Oct 7;286(40):34883-92
pubmed: 21849500
J Immunother. 2013 Feb;36(2):133-51
pubmed: 23377668
J Immunol. 2012 Mar 1;188(5):2102-10
pubmed: 22291187
Cancer Immunol Immunother. 2004 Jun;53(6):510-8
pubmed: 14740176
Blood. 2013 Aug 8;122(6):863-71
pubmed: 23770775
Blood. 2006 Feb 15;107(4):1528-36
pubmed: 16239433
Nat Biotechnol. 2015 Jan;33(1):64-72
pubmed: 25485616
Nat Med. 2000 Dec;6(12):1406-10
pubmed: 11100129
Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3669-74
pubmed: 20133680
Science. 2015 Apr 3;348(6230):62-8
pubmed: 25838374
Nat Biotechnol. 2002 Feb;20(2):143-8
pubmed: 11821859
ACS Chem Biol. 2015 Feb 20;10(2):485-92
pubmed: 25372624