Developing Organoids from Ovarian Cancer as Experimental and Preclinical Models.
ERBB
disease modeling
high-grade serous ovarian cancer
neuregulin-1
organoids
ovarian cancer
Journal
Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300
Informations de publication
Date de publication:
14 04 2020
14 04 2020
Historique:
received:
15
07
2019
revised:
03
03
2020
accepted:
03
03
2020
pubmed:
4
4
2020
medline:
26
3
2021
entrez:
4
4
2020
Statut:
ppublish
Résumé
Ovarian cancer (OC) represents the most dismal gynecological cancer. Pathobiology is poorly understood, mainly due to lack of appropriate study models. Organoids, defined as self-developing three-dimensional in vitro reconstructions of tissues, provide powerful tools to model human diseases. Here, we established organoid cultures from patient-derived OC, in particular from the most prevalent high-grade serous OC (HGSOC). Testing multiple culture medium components identified neuregulin-1 (NRG1) as key factor in maximizing OC organoid development and growth, although overall derivation efficiency remained moderate (36% for HGSOC patients, 44% for all patients together). Established organoid lines showed patient tumor-dependent morphology and disease characteristics, and recapitulated the parent tumor's marker expression and mutational landscape. Moreover, the organoids displayed tumor-specific sensitivity to clinical HGSOC chemotherapeutic drugs. Patient-derived OC organoids provide powerful tools for the study of the cancer's pathobiology (such as importance of the NRG1/ERBB pathway) as well as advanced preclinical tools for (personalized) drug screening and discovery.
Identifiants
pubmed: 32243841
pii: S2213-6711(20)30094-1
doi: 10.1016/j.stemcr.2020.03.004
pmc: PMC7160357
pii:
doi:
Substances chimiques
Antineoplastic Agents
0
Neuregulin-1
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
717-729Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
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