An innovative approach of priming lignocellulosics with lytic polysaccharide mono-oxygenases prior to saccharification with glycosyl hydrolases can economize second generation ethanol process.
Enhanced saccharification
Heterologous expression
Lytic polysaccharide monooxygenases (LPMOs)
Priming with LPMO
Purification
Journal
Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
21
01
2020
revised:
23
03
2020
accepted:
24
03
2020
pubmed:
4
4
2020
medline:
29
4
2020
entrez:
4
4
2020
Statut:
ppublish
Résumé
Two Lytic polysaccharide Mono-Oxygenases (LPMOs), non-modular (PMO_08942) and modular (PMO_07920), from thermotolerant fungus Aspergillus terreus 9DR cloned and expressed in Pichia pastoris X33 and purified to homogeneity using ion-exchange chromatography were found to be of ~29 and ~40 kDa, respectively. Both LPMOs were optimally active at 50 °C; PMO_08942 was active under acidic condition (pH 5.0) and PMO_07920 at pH 7.0. Modular LPMO (PMO_07920) tethered to CBM-1 was found to be versatile as it showed appreciable activity on complex polysaccharide (both cellulose and xylans) as compared to non-modular (PMO_08942). The t
Identifiants
pubmed: 32244131
pii: S0960-8524(20)30528-9
doi: 10.1016/j.biortech.2020.123257
pii:
doi:
Substances chimiques
Polysaccharides
0
lignocellulose
11132-73-3
Ethanol
3K9958V90M
Lignin
9005-53-2
Mixed Function Oxygenases
EC 1.-
Oxygenases
EC 1.13.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
123257Informations de copyright
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