Nine-Year Nationwide Environmental Surveillance of Hepatitis E Virus in Urban Wastewaters in Italy (2011-2019).
PCR
hepatitis E virus
prevalence
sequencing
sewage
typing
Journal
International journal of environmental research and public health
ISSN: 1660-4601
Titre abrégé: Int J Environ Res Public Health
Pays: Switzerland
ID NLM: 101238455
Informations de publication
Date de publication:
20 03 2020
20 03 2020
Historique:
received:
27
02
2020
revised:
18
03
2020
accepted:
18
03
2020
entrez:
5
4
2020
pubmed:
5
4
2020
medline:
22
9
2020
Statut:
epublish
Résumé
Hepatitis E virus (HEV) is an emerging causative agent of acute hepatitis worldwide. To provide insights into the epidemiology of HEV in Italy, a large-scale investigation was conducted into urban sewage over nine years (2011-2019), collecting 1374 sewage samples from 48 wastewater treatment plants located in all the 20 regions of Italy. Broadly reactive primers targeting the ORF1 and ORF2 regions were used for the detection and typing of HEV, followed by Sanger and next generation sequencing (NGS). Real-time RT-qPCR was also used to attempt quantification of positive samples. HEV RNA detection occurred in 74 urban sewage samples (5.4%), with a statistically significant higher frequency (7.1%) in central Italy. Fifty-six samples were characterized as G3 strains and 18 as G1. While the detection of G3 strains occurred in all the surveillance period, G1 strains were mainly detected in 2011-2012, and never in 2017-2019. Typing was achieved in 2 samples (3f subtype). Viral concentrations in quantifiable samples ranged from 1.2 × 10
Identifiants
pubmed: 32244915
pii: ijerph17062059
doi: 10.3390/ijerph17062059
pmc: PMC7143501
pii:
doi:
Substances chimiques
RNA, Viral
0
Waste Water
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Références
Transbound Emerg Dis. 2017 Oct;64(5):1443-1453
pubmed: 27380833
Rev Med Virol. 2003 May-Jun;13(3):145-54
pubmed: 12740830
J Environ Manage. 2015 Aug 15;159:58-67
pubmed: 26046988
J Water Health. 2009 Dec;7(4):664-73
pubmed: 19590134
Bioinformatics. 2007 Feb 15;23(4):500-1
pubmed: 17158514
Food Environ Virol. 2016 Sep;8(3):194-9
pubmed: 27165600
Ann Agric Environ Med. 2018 Jun 20;25(2):364-367
pubmed: 29936796
Arch Virol. 2015 Oct;160(10):2547-56
pubmed: 26215443
Arch Virol. 2012 Dec;157(12):2363-8
pubmed: 22847755
Food Environ Virol. 2015 Dec;7(4):316-24
pubmed: 26115693
Int J Food Microbiol. 2015 Jan 16;193:29-33
pubmed: 25462920
Biochem Biophys Res Commun. 2001 Dec 21;289(5):929-36
pubmed: 11741279
Ann Agric Environ Med. 2015;22(4):661-4
pubmed: 26706972
Vet Microbiol. 2008 Nov 25;132(1-2):47-55
pubmed: 18538512
Food Environ Virol. 2015 Sep;7(3):213-23
pubmed: 26003323
Water Res. 2019 Mar 15;151:144-157
pubmed: 30594083
Food Environ Virol. 2015 Sep;7(3):206-12
pubmed: 26006251
J Gen Virol. 2016 Mar;97(3):537-542
pubmed: 26743685
BMC Res Notes. 2012 Jun 15;5:297
pubmed: 22704073
J Virol Methods. 2012 Dec;186(1-2):157-60
pubmed: 22871672
Vet Microbiol. 2011 May 5;149(3-4):330-8
pubmed: 21216541
Int J Mol Sci. 2017 Apr 20;18(4):
pubmed: 28425927
Transbound Emerg Dis. 2019 May;66(3):1120-1125
pubmed: 30811818
Am J Trop Med Hyg. 2016 Jul 6;95(1):216-20
pubmed: 27246446
Transbound Emerg Dis. 2019 Sep;66(5):1803-1808
pubmed: 31127865
Euro Surveill. 2016 Jul 28;21(30):
pubmed: 27494608
New Microbiol. 2013 Oct;36(4):331-44
pubmed: 24177295
Liver Int. 2016 Jan;36 Suppl 1:130-4
pubmed: 26725910
J Med Virol. 2016 May;88(5):915-8
pubmed: 26461450
Emerg Infect Dis. 2003 Apr;9(4):448-54
pubmed: 12702225
Epidemiol Infect. 2015 Feb;143(3):529-33
pubmed: 24831185
Emerg Infect Dis. 2013 Jan;19(1):110-4
pubmed: 23260079
J Med Virol. 2009 Oct;81(10):1743-9
pubmed: 19697406
J Gen Virol. 2011 Jul;92(Pt 7):1617-1626
pubmed: 21471314
J Med Virol. 1994 Mar;42(3):318-20
pubmed: 8006645
Food Environ Virol. 2017 Mar;9(1):79-88
pubmed: 27682315
Food Environ Virol. 2017 Jun;9(2):219-229
pubmed: 27853931
Int J Infect Dis. 2019 Mar;80:66-72
pubmed: 30639406
J Med Virol. 2003 Feb;69(2):207-14
pubmed: 12683409
Virol J. 2014 Apr 22;11:72
pubmed: 24755361
Water Res. 2013 Sep 15;47(14):5101-9
pubmed: 23866141
Int J Hyg Environ Health. 2018 Apr;221(3):479-488
pubmed: 29402695
J Viral Hepat. 2019 Apr;26(4):506-512
pubmed: 30548124
Epidemiol Infect. 2014 Feb;142(2):287-94
pubmed: 23673019
Appl Environ Microbiol. 2010 Sep;76(17):5870-3
pubmed: 20601505
Virol J. 2011 Apr 27;8:195
pubmed: 21524296
BMC Vet Res. 2014 Jun 16;10:133
pubmed: 24934984
Transbound Emerg Dis. 2018 Dec;65(6):1749-1756
pubmed: 30207081
Water Res. 2017 Sep 15;121:240-247
pubmed: 28550812
J Clin Pathol. 2017 Aug 9;:
pubmed: 28794125
Travel Med Infect Dis. 2017 Nov - Dec;20:37-42
pubmed: 28411095
BMC Infect Dis. 2014 Jul 29;14:419
pubmed: 25074676
Clin Microbiol Infect. 2011 Aug;17(8):1182-5
pubmed: 21375654
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Zoonoses Public Health. 2008 Aug;55(6):291-8
pubmed: 18638181
Euro Surveill. 2018 Oct;23(41):
pubmed: 30326991
Infez Med. 2015 Sep;23(3):253-6
pubmed: 26397295
Res Virol. 1998 Sep-Oct;149(5):271-6
pubmed: 9879604
Eur J Clin Microbiol Infect Dis. 2017 Mar;36(3):479-485
pubmed: 27785638
Front Microbiol. 2017 Feb 15;8:230
pubmed: 28261185
J Virol Methods. 2006 Jan;131(1):65-71
pubmed: 16125257
Food Environ Virol. 2019 Dec;11(4):420-426
pubmed: 31512058
J Clin Virol. 2014 Nov;61(3):334-9
pubmed: 25213209
Bioinformatics. 2014 Jul 1;30(13):1928-9
pubmed: 24618473
J Clin Transl Hepatol. 2015 Jun 28;3(2):117-26
pubmed: 26355220
Food Microbiol. 2019 Sep;82:194-201
pubmed: 31027773
J Gen Virol. 2017 Nov;98(11):2645-2646
pubmed: 29022866
Food Environ Virol. 2018 Mar;10(1):127-131
pubmed: 28956272
Vet Microbiol. 2008 Jan 1;126(1-3):74-81
pubmed: 17706898
Blood Transfus. 2018 Sep;16(5):413-421
pubmed: 29757135
J Water Health. 2010 Jun;8(2):346-54
pubmed: 20154397
Res Vet Sci. 2010 Jun;88(3):492-6
pubmed: 20092862
Arch Virol. 2015 Jan;160(1):183-9
pubmed: 25307960
J Hepatol. 2008 Mar;48(3):494-503
pubmed: 18192058
Gastroenterology. 2016 Feb;150(2):355-7.e3
pubmed: 26551551
Open Forum Infect Dis. 2019 Jun 26;6(9):ofz306
pubmed: 31660396
J Viral Hepat. 2013 Jun;20(6):438-43
pubmed: 23647961
J Gastroenterol Hepatol. 2011 Jan;26 Suppl 1:72-82
pubmed: 21199517