A genome-wide CRISPR screen identifies regulation factors of the TLR3 signalling pathway.
Cells, Cultured
Chemokine CXCL10
/ metabolism
Clustered Regularly Interspaced Short Palindromic Repeats
Gene Expression Regulation
Humans
Immunity, Innate
Interferon Regulatory Factor-3
/ genetics
Interleukin-8
/ metabolism
Macrophages
/ immunology
Membrane Transport Proteins
/ genetics
NF-kappa B
/ genetics
Poly I-C
/ immunology
Receptors, Aryl Hydrocarbon
/ genetics
Signal Transduction
Toll-Like Receptor 3
/ genetics
AhR
TLR
TLR3
genetic screen
innate immunity
Journal
Innate immunity
ISSN: 1753-4267
Titre abrégé: Innate Immun
Pays: United States
ID NLM: 101469670
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
pubmed:
7
4
2020
medline:
1
10
2021
entrez:
7
4
2020
Statut:
ppublish
Résumé
A subset of TLRs is specialised in the detection of incoming pathogens by sampling endosomes for nucleic acid contents. Among them, TLR3 senses the abnormal presence of double-stranded RNA in the endosomes and initiates a potent innate immune response via activation of NF-κB and IRF3. Nevertheless, mechanisms governing TLR3 regulation remain poorly defined. To identify new molecular players involved in the TLR3 pathway, we performed a genome-wide screen using CRISPR/Cas9 technology. We generated TLR3
Identifiants
pubmed: 32248720
doi: 10.1177/1753425920915507
pmc: PMC7491238
doi:
Substances chimiques
CXCL10 protein, human
0
Chemokine CXCL10
0
IRF3 protein, human
0
Interferon Regulatory Factor-3
0
Interleukin-8
0
Membrane Transport Proteins
0
NF-kappa B
0
Receptors, Aryl Hydrocarbon
0
Toll-Like Receptor 3
0
UNC93B1 protein, human
0
Poly I-C
O84C90HH2L
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
459-472Subventions
Organisme : Wellcome Trust
ID : 210688/Z/18/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/V011561/1
Pays : United Kingdom
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