Skin wipe test: A simple, inexpensive, and fast approach in the diagnosis of cystic fibrosis.
capillary electrophoresis
cystic fibrosis
diagnosis
ion ratio
screening
skin wipe test
sweat test
Journal
Pediatric pulmonology
ISSN: 1099-0496
Titre abrégé: Pediatr Pulmonol
Pays: United States
ID NLM: 8510590
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
06
11
2019
revised:
04
03
2020
accepted:
22
03
2020
pubmed:
7
4
2020
medline:
3
11
2020
entrez:
7
4
2020
Statut:
ppublish
Résumé
To assess the performance of a newly developed skin wipe test (SWT) for the diagnosis of cystic fibrosis (CF). Spontaneously formed sweat from the forearm was wiped by a cotton swab moistened with 100 µL of deionized (DI) water and extracted into 400 µL of DI water (SWT). The conventional Macroduct sweat test (ST) was performed simultaneously. SWT samples of 114 CF patients, 76 healthy carriers, and 58 controls were analyzed by capillary electrophoresis with contactless conductivity detection and Cl Analysis of 248 SWT samples and simultaneous Macroduct ST samples showed comparable method performance. Two ion ratios, Cl The developed SWT method with capillary electrophoretic analysis for CF diagnosis performs comparably to the conventional Macroduct ST. The SWT method is simple, fast, inexpensive, and completely noninvasive. Use of an ion ratio in obtained SWT samples is proposed as a new diagnostic parameter that shows significant promise in CF diagnostics.
Substances chimiques
Chlorides
0
Sodium
9NEZ333N27
Potassium
RWP5GA015D
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1653-1660Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 Wiley Periodicals, Inc.
Références
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