An Oncometabolite Isomer Rapidly Induces a Pathophysiological Protein Modification.
Acylation
Cell Line, Tumor
Cysteine
/ chemistry
Fumarates
/ chemistry
HEK293 Cells
Humans
Hydrogen-Ion Concentration
Maleates
/ chemistry
Phenols
/ chemistry
Protein Processing, Post-Translational
/ drug effects
Proteome
/ chemistry
Proteomics
/ methods
Succinates
/ chemistry
Sulfhydryl Compounds
/ chemistry
Journal
ACS chemical biology
ISSN: 1554-8937
Titre abrégé: ACS Chem Biol
Pays: United States
ID NLM: 101282906
Informations de publication
Date de publication:
17 04 2020
17 04 2020
Historique:
pubmed:
7
4
2020
medline:
23
1
2021
entrez:
7
4
2020
Statut:
ppublish
Résumé
Metabolites regulate protein function via covalent and noncovalent interactions. However, manipulating these interactions in living cells remains a major challenge. Here, we report a chemical strategy for inducing cysteine S-succination, a nonenzymatic post-translational modification derived from the oncometabolite fumarate. Using a combination of antibody-based detection and kinetic assays, we benchmark the
Identifiants
pubmed: 32250583
doi: 10.1021/acschembio.0c00044
pmc: PMC8453589
mid: NIHMS1733118
doi:
Substances chimiques
Fumarates
0
Maleates
0
Phenols
0
Proteome
0
Succinates
0
Sulfhydryl Compounds
0
thiophenol
7K011JR4T0
maleic acid
91XW058U2C
Cysteine
K848JZ4886
bromosuccinic acid
O3K54IBV7F
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
856-861Subventions
Organisme : Intramural NIH HHS
ID : ZIA BC011488
Pays : United States
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