A novel endoscopic visible full-thickness cryoablation device on stomach.
Animals
Apoptosis
Cell Culture Techniques
Cell Line
Cicatrix
/ pathology
Cold Temperature
Collagen
Cryosurgery
/ instrumentation
Drug Combinations
Equipment Design
Gastric Mucosa
/ pathology
Gastroscopes
Gastroscopy
/ instrumentation
Humans
Laminin
Proteoglycans
Stomach
/ pathology
Swine
Temperature
Tissue Adhesions
Wound Healing
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 03 2020
23 03 2020
Historique:
received:
25
07
2019
accepted:
17
02
2020
entrez:
7
4
2020
pubmed:
7
4
2020
medline:
1
12
2020
Statut:
epublish
Résumé
Cryoablation has been used for the treatment of various sorts of solid visceral tumors, but few are reported on gastric tumor via endoscope, in terms of accurate control of ablation site, freezing depth and effective temperature. Thus, we developed a novel device, which could perform accurate cryoablation on the stomach via endoscope. This study aimed to evaluate the efficacy and safety of the device on porcine stomach. Results showed that the novel device could provide direct view of the operation space, allowing accurate and safe ablation of the stomach. Three minutes cryoablation caused a transmural, 1 cm radius gastric lesion. On serosal side, the temperature dropped to -64.2 °C, -34.1 °C, 26.1 °C at the center, 1 cm and 2 cm from center, respectively. Histopathology revealed acute ruptured cells with damaged glands in mucosa, partial disruption in muscularis propria and serosal slight exudation. Three months later, scar formed with complete recovery of gastric structure. No active bleeding or perforation of stomach, nor injury or adhesion of adjacent organs was observed. This endoscopic cryoablation device allowed safe, full-thickness cryoablation with effective temperature, which may provide an alternative treatment for gastric tumor.
Identifiants
pubmed: 32251315
doi: 10.1038/s41598-020-61595-x
pii: 10.1038/s41598-020-61595-x
pmc: PMC7090056
doi:
Substances chimiques
Drug Combinations
0
Laminin
0
Proteoglycans
0
matrigel
119978-18-6
Collagen
9007-34-5
Types de publication
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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