Genetic tool development in marine protists: emerging model organisms for experimental cell biology.
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
26
06
2019
accepted:
02
03
2020
pubmed:
7
4
2020
medline:
19
8
2020
entrez:
7
4
2020
Statut:
ppublish
Résumé
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.
Identifiants
pubmed: 32251396
doi: 10.1038/s41592-020-0796-x
pii: 10.1038/s41592-020-0796-x
pmc: PMC7200600
doi:
Substances chimiques
Green Fluorescent Proteins
147336-22-9
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
481-494Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM040478
Pays : United States
Commentaires et corrections
Type : CommentIn
Type : ErratumIn
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