Multiplex Genetic Engineering Exploiting Pyrimidine Salvage Pathway-Based Endogenous Counterselectable Markers.
Animals
Anti-Bacterial Agents
/ pharmacology
Aspergillosis
/ microbiology
Aspergillus fumigatus
/ drug effects
Female
Fusarium
/ drug effects
Genetic Engineering
/ methods
Genetic Markers
Humans
Mice
Mutagenesis, Insertional
Penicillium chrysogenum
/ drug effects
Pyrimidines
/ metabolism
Specific Pathogen-Free Organisms
endogenous selectable markers
genetic engineering
pyrimidine salvage pathway
targeted genomic integrations
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
07 04 2020
07 04 2020
Historique:
entrez:
9
4
2020
pubmed:
9
4
2020
medline:
18
2
2021
Statut:
epublish
Résumé
Selectable markers are indispensable for genetic engineering, yet their number and variety are limited. Most selection procedures for prototrophic cells rely on the introduction of antibiotic resistance genes. New minimally invasive tools are needed to facilitate sophisticated genetic manipulations. Here, we characterized three endogenous genes in the human fungal pathogen
Identifiants
pubmed: 32265325
pii: mBio.00230-20
doi: 10.1128/mBio.00230-20
pmc: PMC7157766
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Genetic Markers
0
Pyrimidines
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Austrian Science Fund FWF
ID : P 31093
Pays : Austria
Informations de copyright
Copyright © 2020 Birštonas et al.
Références
Clin Microbiol Infect. 2008 Oct;14(10):982-4
pubmed: 18828858
Methods Enzymol. 1992;216:447-57
pubmed: 1479914
Crit Rev Microbiol. 1987;15(1):45-56
pubmed: 3319420
Nucleic Acids Res. 2008 Dec;36(22):e151
pubmed: 18984616
Yeast. 2006 Jul 15;23(9):707-15
pubmed: 16845689
Mol Microbiol. 2008 May;68(4):959-77
pubmed: 18384518
Arch Microbiol. 2005 Nov;184(2):93-100
pubmed: 16163515
Mol Gen Genet. 1984;197(2):345-6
pubmed: 6394957
J Microbiol. 2010 Apr;48(2):188-98
pubmed: 20437151
FEMS Microbiol Lett. 2019 Jan 1;366(1):
pubmed: 30561594
PLoS One. 2013 Nov 25;8(11):e81370
pubmed: 24282588
Chemotherapy. 1976;22(3-4):137-53
pubmed: 773604
Adv Genet. 1953;5:141-238
pubmed: 13040135
Proc Natl Acad Sci U S A. 1988 Oct;85(20):7572-6
pubmed: 2845412
Mol Biochem Parasitol. 1999 Jan 5;98(1):93-103
pubmed: 10029312
PLoS One. 2012;7(6):e38262
pubmed: 22675534
FEMS Microbiol Rev. 2014 Sep;38(5):947-95
pubmed: 24724938
Antimicrob Agents Chemother. 2018 May 25;62(6):
pubmed: 29610197
Gene. 1990 Apr 16;88(2):149-57
pubmed: 2189783
Pathogens. 2015 Apr 03;4(2):157-81
pubmed: 25854922
Antimicrob Agents Chemother. 2004 Aug;48(8):3147-50
pubmed: 15273136
J Antimicrob Chemother. 2013 Jul;68(7):1486-96
pubmed: 23580559
J Bacteriol. 1970 Jun;102(3):607-15
pubmed: 5429721
J Med Chem. 1964 Jul;7:574-5
pubmed: 14221156
Biochim Biophys Acta. 2000 Dec 15;1529(1-3):89-102
pubmed: 11111079
J Antimicrob Chemother. 2000 Aug;46(2):171-9
pubmed: 10933638
Curr Genet. 1996 Jun;30(1):76-82
pubmed: 8662213
Mol Microbiol. 2009 Jul;73(1):43-57
pubmed: 19460095
Antimicrob Agents Chemother. 2005 Aug;49(8):3341-6
pubmed: 16048945
Adv Appl Microbiol. 2012;81:1-61
pubmed: 22958526
Malar J. 2012 Mar 31;11:103
pubmed: 22463060
Metallomics. 2012 Dec;4(12):1262-70
pubmed: 23151814
Biosci Biotechnol Biochem. 2002 Feb;66(2):404-6
pubmed: 11999416
Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):33-7
pubmed: 1729703
Cell. 1977 May;11(1):223-32
pubmed: 194704
BMC Biotechnol. 2013 Apr 23;13:36
pubmed: 23617583