Increased extracellular vesicles mediate inflammatory signalling in cystic fibrosis.
Adolescent
Adult
Age Factors
Bronchoalveolar Lavage Fluid
/ chemistry
Cell Line
Cell Movement
Cells, Cultured
Chemotaxis
Child
Child, Preschool
Cystic Fibrosis
/ metabolism
Cystic Fibrosis Transmembrane Conductance Regulator
/ metabolism
Extracellular Vesicles
/ metabolism
Female
Humans
Infant
Male
Mass Spectrometry
Nanoparticles
Neutrophils
/ metabolism
Pilot Projects
Signal Transduction
Transfection
cystic fibrosis
neutrophil biology
paediatric lung disaese
Journal
Thorax
ISSN: 1468-3296
Titre abrégé: Thorax
Pays: England
ID NLM: 0417353
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
30
08
2019
revised:
09
03
2020
accepted:
20
03
2020
pubmed:
9
4
2020
medline:
18
8
2020
entrez:
9
4
2020
Statut:
ppublish
Résumé
Mutations in the cystic fibrosis transmembrane regulator ( To investigate if extracellular vesicles (EVs) contribute to inflammatory signalling in CF. EVs released from CFBE41o-, CuFi-5, 16HBE14o- and NuLi-1 cells were characterised by nanoparticle tracking analysis (NTA). EVs isolated from bronchoalveolar lavage fluid (BALF) from 30 people with CF (PWCF) were analysed by NTA and mass spectrometry and compared with controls. Neutrophils were isolated from the blood of 8 PWCF to examine neutrophil migration in the presence of CFBE41o- EVs. A significantly higher level of EVs were released from CFBE41o- (p<0.0001) and CuFi-5 (p=0.0209) relative to control cell lines. A significantly higher level of EVs were detected in BALF of PWCF, in three different age groups relative to controls (p=0.01, 0.001, 0.002). A significantly lower level of EVs were released from CFBE41o- (p<0.001) and CuFi-5 (p=0.0002) cell lines treated with CFTR modulators. Significant changes in the protein expression of 126 unique proteins was determined in EVs obtained from the BALF of PWCF of different age groups (p<0.001-0.05). A significant increase in chemotaxis of neutrophils derived from PWCF was observed in the presence of CFBE41o EVs (p=0.0024) compared with controls. This study demonstrates that EVs are produced in CF airway cells, have differential protein expression at different ages and drive neutrophil recruitment in CF.
Identifiants
pubmed: 32265339
pii: thoraxjnl-2019-214027
doi: 10.1136/thoraxjnl-2019-214027
pmc: PMC7279202
doi:
Substances chimiques
Cystic Fibrosis Transmembrane Conductance Regulator
126880-72-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
449-458Informations de copyright
© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
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