Increased expression of immune checkpoint programmed cell death protein-1 (PD-1) on T cell subsets of bone marrow aspirates in patients with B-Lymphoblastic leukemia, especially in relapse and at diagnosis.
Adult
Apoptosis Regulatory Proteins
/ immunology
B7-H1 Antigen
/ genetics
CD8-Positive T-Lymphocytes
/ immunology
Female
Flow Cytometry
Gene Expression Regulation, Leukemic
/ genetics
Humans
Immune Checkpoint Inhibitors
/ immunology
Male
Middle Aged
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma
/ diagnosis
Programmed Cell Death 1 Ligand 2 Protein
/ genetics
Programmed Cell Death 1 Receptor
/ genetics
Recurrence
T-Lymphocyte Subsets
/ immunology
B-lymphoblastic leukemia
T cell subsets
flow cytometry
immune checkpoint PD-1
Journal
Cytometry. Part B, Clinical cytometry
ISSN: 1552-4957
Titre abrégé: Cytometry B Clin Cytom
Pays: United States
ID NLM: 101235690
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
20
08
2019
revised:
24
03
2020
accepted:
26
03
2020
pubmed:
9
4
2020
medline:
24
8
2021
entrez:
9
4
2020
Statut:
ppublish
Résumé
We analyzed expression profiles of immune checkpoint receptors on T cell subsets and ligands on leukemic blasts in patients with B-lymphoblastic leukemia (B-ALL). Total 149 bone marrow (BM) samples obtained from 65 B-ALL patients with four different clinical status (41 at diagnosis, 54 in complete remission [CR], 34 in persistence, and 20 in relapse), and 32 BM control samples were prospectively enrolled. Expression of immune checkpoint receptor (programmed cell death protein-1 [PD-1]) on T cell subsets and ligands (PD-L1, PD-L2) on leukemic blasts was evaluated by flow cytometry, and was compared between patient subgroups. Relapsed patients demonstrated highest PD-1 expression proportion and intensity on CD3 In BM aspirates from B-ALL patients, PD-1 expression on T-cell subsets is increased at diagnosis, and to a greater extent, at relapse. These data suggest the potential usefulness of PD-1 blockade in the treatment of B-ALL, particularly at relapse.
Sections du résumé
BACKGROUND
We analyzed expression profiles of immune checkpoint receptors on T cell subsets and ligands on leukemic blasts in patients with B-lymphoblastic leukemia (B-ALL).
METHODS
Total 149 bone marrow (BM) samples obtained from 65 B-ALL patients with four different clinical status (41 at diagnosis, 54 in complete remission [CR], 34 in persistence, and 20 in relapse), and 32 BM control samples were prospectively enrolled. Expression of immune checkpoint receptor (programmed cell death protein-1 [PD-1]) on T cell subsets and ligands (PD-L1, PD-L2) on leukemic blasts was evaluated by flow cytometry, and was compared between patient subgroups.
RESULTS
Relapsed patients demonstrated highest PD-1 expression proportion and intensity on CD3
CONCLUSIONS
In BM aspirates from B-ALL patients, PD-1 expression on T-cell subsets is increased at diagnosis, and to a greater extent, at relapse. These data suggest the potential usefulness of PD-1 blockade in the treatment of B-ALL, particularly at relapse.
Identifiants
pubmed: 32268011
doi: 10.1002/cyto.b.21879
doi:
Substances chimiques
Apoptosis Regulatory Proteins
0
B7-H1 Antigen
0
CD274 protein, human
0
Immune Checkpoint Inhibitors
0
PDCD1 protein, human
0
PDCD1LG2 protein, human
0
Programmed Cell Death 1 Ligand 2 Protein
0
Programmed Cell Death 1 Receptor
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
336-347Informations de copyright
© 2020 International Clinical Cytometry Society.
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