Plasma Cell Fate Is Orchestrated by Elaborate Changes in Genome Compartmentalization and Inter-chromosomal Hubs.
Activating Transcription Factor 4
/ genetics
Animals
Cell Differentiation
/ genetics
Cell Lineage
/ physiology
Chromosomes
/ genetics
Epigenesis, Genetic
/ genetics
Female
Genome
/ genetics
Heterochromatin
/ genetics
Histones
/ genetics
Male
Mice
Mice, Inbred C57BL
Plasma Cells
/ cytology
Positive Regulatory Domain I-Binding Factor 1
/ genetics
Regulatory Sequences, Nucleic Acid
/ genetics
Trans-Activators
/ genetics
Transcription Factors
/ metabolism
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
07 04 2020
07 04 2020
Historique:
received:
20
11
2019
revised:
31
01
2020
accepted:
12
03
2020
entrez:
9
4
2020
pubmed:
9
4
2020
medline:
28
4
2021
Statut:
ppublish
Résumé
The transition from the follicular B to the plasma cell stage is associated with large-scale changes in cell morphology. Here, we examine whether plasma cell development is also associated with changes in nuclear architecture. We find that the onset of plasma cell development is concomitant with a decline in remote genomic interactions; a gain in euchromatic character at loci encoding for factors that specify plasma cell fate, including Prdm1 and Atf4; and establishment of de novo inter-chromosomal hubs. We find that, in developing plasma cells and concurrent with transcriptional silencing, the Ebf1 locus repositions from an euchromatic to peri-centromeric heterochromatic environment. Finally, we find that inter-chromosomal hubs are enriched for the deposition of either H3K27Ac or H3K27me3. These data indicate that plasma cell fate is orchestrated by elaborate changes in genome topology and that epigenetic marks, linked with super-enhancers or transcriptionally repressed regions, are enriched at inter-chromosomal hubs.
Identifiants
pubmed: 32268089
pii: S2211-1247(20)30348-X
doi: 10.1016/j.celrep.2020.03.034
pii:
doi:
Substances chimiques
Atf4 protein, mouse
0
Ebf1 protein, mouse
0
Heterochromatin
0
Histones
0
Trans-Activators
0
Transcription Factors
0
Activating Transcription Factor 4
145891-90-3
Positive Regulatory Domain I-Binding Factor 1
EC 2.1.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107470Subventions
Organisme : NIGMS NIH HHS
ID : F32 GM106631
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2020 University of California, San Diego. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.