Impact of Atrazine Exposure on the Microbial Community Structure in a Brazilian Tropical Latosol Soil.
Atrazine
/ pharmacology
Biodegradation, Environmental
Brazil
Genes, Bacterial
Gram-Negative Bacteria
/ classification
Gram-Positive Bacteria
/ classification
Herbicides
/ pharmacology
Microbiota
/ drug effects
RNA, Ribosomal, 16S
/ genetics
Soil
/ chemistry
Soil Microbiology
Soil Pollutants
/ pharmacology
Tropical Climate
16S rRNA gene sequencing
atzA
atzD
qPCR
trzN
Journal
Microbes and environments
ISSN: 1347-4405
Titre abrégé: Microbes Environ
Pays: Japan
ID NLM: 9710937
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
10
4
2020
pubmed:
10
4
2020
medline:
10
10
2020
Statut:
ppublish
Résumé
Atrazine is a triazine herbicide that is widely used to control broadleaf weeds. Its widespread use over the last 50 years has led to the potential contamination of soils, groundwater, rivers, and lakes. Its main route of complete degradation is via biological means, which is carried out by soil microbiota using a 6-step pathway. The aim of the present study was to investigate whether application of atrazine to soil changes the soil bacterial community. We used 16S rRNA gene sequencing and qPCR to elucidate the microbial community structure and assess the abundance of the atrazine degradation genes atzA, atzD, and trzN in a Brazilian soil. The results obtained showed that the relative abundance of atzA and trzN, encoding triazine-initiating metabolism in Gram-negative and -positive bacteria, respectively, increased in soil during the first weeks following the application of atrazine. In contrast, the abundance of atzD, encoding cyanuric acid amidohydrolase-the fourth step in the pathway-was not related to the atrazine treatment. Moreover, the overall soil bacterial community showed no significant changes after the application of atrazine. Despite this, we observed increases in the relative abundance of bacterial families in the 4
Identifiants
pubmed: 32269200
doi: 10.1264/jsme2.ME19143
pmc: PMC7308567
doi:
Substances chimiques
Herbicides
0
RNA, Ribosomal, 16S
0
Soil
0
Soil Pollutants
0
Atrazine
QJA9M5H4IM
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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