Characterization of Vesicle Differentiation Mutants of Frankia casuarinae.
glutamine synthetase
multicellular bacteria
nitrogen fixation
vesicle
Journal
Microbes and environments
ISSN: 1347-4405
Titre abrégé: Microbes Environ
Pays: Japan
ID NLM: 9710937
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
10
4
2020
pubmed:
10
4
2020
medline:
10
10
2020
Statut:
ppublish
Résumé
The nitrogen-fixing actinobacterium Frankia develops unique multicellular structures called vesicles, which are the site of nitrogen fixation. These vesicles are surrounded by a thick hopanoid lipid envelope that protects nitrogenase against oxygen inactivation. The phenotypes of five mutants that form smaller numbers of vesicles were investigated. The vesicles of these mutants were smaller than those of the wild type and had a phase dark appearance. They induced the expression of a glutamine synthetase gene in hyphae cells in response to ammonium starvation. These results suggest that genes impaired in the mutants do not function in global nitrogen regulation, but specifically function in vesicle differentiation.
Identifiants
pubmed: 32269204
doi: 10.1264/jsme2.ME19150
pmc: PMC7308572
doi:
Substances chimiques
Ammonium Compounds
0
Bacterial Proteins
0
Glutamate-Ammonia Ligase
EC 6.3.1.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
Microbiol Rev. 1995 Dec;59(4):604-22
pubmed: 8531888
Appl Environ Microbiol. 2019 Jul 18;85(15):
pubmed: 31152017
Microbiol Rev. 1993 Jun;57(2):293-319
pubmed: 8336669
Microbes Environ. 2017 Dec 27;32(4):344-351
pubmed: 29151446
Front Microbiol. 2019 Sep 24;10:2230
pubmed: 31608043
Microbes Environ. 2009;24(3):231-40
pubmed: 21566378
Plant Physiol. 1987 Apr;83(4):728-31
pubmed: 16665327
J Bacteriol. 1990 Mar;172(3):1380-4
pubmed: 1968454
Int J Syst Evol Microbiol. 2016 Dec;66(12):5201-5210
pubmed: 27624710
Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6091-4
pubmed: 11607408
Nat Rev Microbiol. 2004 Aug;2(8):621-31
pubmed: 15263897
Can J Microbiol. 1985 Sep;31(9):804-9
pubmed: 3866636
Biomed Res Int. 2014;2014:568549
pubmed: 24987692