Cep55 promotes cytokinesis of neural progenitors but is dispensable for most mammalian cell divisions.
Animals
Animals, Newborn
Apoptosis
Cell Cycle Proteins
/ deficiency
Cells, Cultured
Cerebral Cortex
/ abnormalities
Cytokinesis
Endosomal Sorting Complexes Required for Transport
/ metabolism
Fibroblasts
/ metabolism
Gene Deletion
Genotype
Kidney
/ abnormalities
Mice, Inbred C57BL
Mice, Knockout
Microcephaly
/ pathology
Mitosis
Neural Stem Cells
/ cytology
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
08 04 2020
08 04 2020
Historique:
received:
01
04
2019
accepted:
27
02
2020
entrez:
10
4
2020
pubmed:
10
4
2020
medline:
28
7
2020
Statut:
epublish
Résumé
In mammalian cell lines, the endosomal sorting complex required for transport (ESCRT)-III mediates abscission, the process that physically separates daughter cells and completes cell division. Cep55 protein is regarded as the master regulator of abscission, because it recruits ESCRT-III to the midbody (MB), the site of abscission. However, the importance of this mechanism in a mammalian organism has never been tested. Here we show that Cep55 is dispensable for mouse embryonic development and adult tissue homeostasis. Cep55-knockout offspring show microcephaly and primary neural progenitors require Cep55 and ESCRT for survival and abscission. However, Cep55 is dispensable for cell division in embryonic or adult tissues. In vitro, division of primary fibroblasts occurs without Cep55 and ESCRT-III at the midbody and is not affected by ESCRT depletion. Our work defines Cep55 as an abscission regulator only in specific tissue contexts and necessitates the re-evaluation of an alternative ESCRT-independent cell division mechanism.
Identifiants
pubmed: 32269212
doi: 10.1038/s41467-020-15359-w
pii: 10.1038/s41467-020-15359-w
pmc: PMC7142149
doi:
Substances chimiques
CHMP4B protein, mouse
0
Cell Cycle Proteins
0
Cep55 protein, mouse
0
Endosomal Sorting Complexes Required for Transport
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1746Subventions
Organisme : Cancer Research UK
ID : FC001039
Pays : United Kingdom
Organisme : Wellcome Trust
ID : FC001039
Pays : United Kingdom
Organisme : Medical Research Council
ID : FC001039
Pays : United Kingdom
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