Gelatin hydrogels with eicosapentaenoic acid can prevent osteoarthritis progression in vivo in a mouse model.
Animals
Biomarkers
/ metabolism
Cartilage, Articular
/ metabolism
Disease Progression
Drug Evaluation, Preclinical
Eicosapentaenoic Acid
/ administration & dosage
Gelatin
Humans
Hydrogels
Male
Mice, Inbred C57BL
Micelles
Osteoarthritis
/ drug therapy
Primary Cell Culture
Random Allocation
Synovitis
/ drug therapy
eicosapentaenoic acid
gelatin hydrogel
omega-3 polyunsaturated fatty acid
osteoarthritis
Journal
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
ISSN: 1554-527X
Titre abrégé: J Orthop Res
Pays: United States
ID NLM: 8404726
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
07
02
2020
revised:
05
04
2020
accepted:
06
04
2020
pubmed:
10
4
2020
medline:
15
12
2020
entrez:
10
4
2020
Statut:
ppublish
Résumé
Eicosapentanoic acid (EPA) is an antioxidant and omega-3 polyunsaturated fatty acid that reduces inflammatory cytokine production. Gelatin hydrogel can be used as a carrier of a physiologically active substance that release it gradually for an average of ~3 weeks. Therefore, this study aimed to clarify the effect of EPA-incorporating gelatin hydrogels on osteoarthritis (OA) progression in vivo. Ten-week-old male C57BL/6J mice were randomly divided into six groups (n = 6): Sham, destabilization of the medial meniscus (DMM), Corn: DMM + 2 µL corn oil, EPA injection alone (EPA-I): DMM + 2 µL corn oil + 125 μg/μL EPA, Gel: DMM + gelatin hydrogels, and EPA-G: DMM + 125 μg/μL EPA-incorporating gelatin hydrogels. The mice were euthanized at 8 weeks after DMM or Sham surgery, and subjected to histological evaluation. Matrix-metalloproteinases-3 (MMP-3), MMP-13, interleukin-1β (IL-1β), p-IKK α/β, CD86, and CD163 protein expression in the synovial cartilage was detected by immunohistochemical staining. F4/80 expression was also assessed using the F4/80 score of macrophage. Histological score was significantly lower in EPA-G than in EPA-I. MMP-3-, MMP-13-, IL-1β-, and p-IKK α/β-positive cell ratio was significantly lower in EPA-G than in EPA-I. However, CD86- and CD163-positive cell ratio was not significantly different between EPA-I and EPA-G. The average-sum F4/80 score of macrophage in EPA-G was significantly lower than that in EPA-I. EPA-incorporating gelatin hydrogels were shown to prevent OA progression in vivo more effectively than EPA injection alone. Our results suggested that intra-articular administration of controlled-release EPA can be a new therapeutic approach for treating OA.
Substances chimiques
Biomarkers
0
Hydrogels
0
Micelles
0
Gelatin
9000-70-8
Eicosapentaenoic Acid
AAN7QOV9EA
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2157-2169Informations de copyright
© 2020 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
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