Biolistic DNA Delivery in Maize Immature Embryos.

Biolistics / methods DNA, Plant / genetics Gold Osmosis Plants, Genetically Modified Regeneration Zea mays / embryology
Gene gun Genetic transformation Hi II genotype In vitro Type-II callus Zea mays

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 12 4 2020
pubmed: 12 4 2020
medline: 4 2 2021
Statut: ppublish

Résumé

One of the key factors for ensuring a successful genetic transformation is to effectively introduce genetic materials, such as plasmid DNA, into plant cells. A biolistic gun is one of the two best established and most popular tools for delivery of DNA into maize cells. It is the method that generated the first fertile transgenic maize plant. In this chapter, we describe steps involved in introducing single or paired plasmid DNAs into immature embryos of maize Hi II hybrid genotype, using Biolistic

Identifiants

DOI: 10.1007/978-1-0716-0356-7_9 PMID: 32277454
pubmed: 32277454
doi: 10.1007/978-1-0716-0356-7_9
doi:

Substances chimiques

DNA, Plant 0
Gold 7440-57-5

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

177-195

Auteurs

Kan Wang (K)

Department of Agronomy and Crop Bioengineering Center, Iowa State University, Ames, IA, USA. kanwang@iastate.edu.

Huilan Zhu (H)

Department of Agronomy and Crop Bioengineering Center, Iowa State University, Ames, IA, USA.

Morgan McCaw (M)

Department of Agronomy and Crop Bioengineering Center, Iowa State University, Ames, IA, USA.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de transfert de gènes › Biolistique › Biolistic DNA Delivery in Maize Immature Embryos.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN des plantes › Biolistic DNA Delivery in Maize Immature Embryos.
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Or › Biolistic DNA Delivery in Maize Immature Embryos.
questionsmedicales.fr › Phénomènes chimiques › Perméabilité › Osmose › Biolistic DNA Delivery in Maize Immature Embryos.
questionsmedicales.fr › Stades d'organismes › Organismes génétiquement modifiés › Végétaux génétiquement modifiés › Biolistic DNA Delivery in Maize Immature Embryos.
questionsmedicales.fr › Phénomènes biologiques › Régénération › Biolistic DNA Delivery in Maize Immature Embryos.
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Zea mays › Biolistic DNA Delivery in Maize Immature Embryos.