Expressions of ezrin, ERK, STAT3, and AKT in tongue cancer and association with tumor characteristics and patient survival.


Journal

Clinical and experimental dental research
ISSN: 2057-4347
Titre abrégé: Clin Exp Dent Res
Pays: United States
ID NLM: 101692332

Informations de publication

Date de publication:
08 2020
Historique:
received: 21 10 2019
revised: 07 02 2020
accepted: 06 03 2020
pubmed: 14 4 2020
medline: 24 8 2021
entrez: 14 4 2020
Statut: ppublish

Résumé

Ezrin, ERK, STAT3, and AKT are proteins that are overexpressed in various types of cancer, although their expressions in tongue cancer has received less focus. This study aimed to address associations between the expression levels of these proteins and with characteristics of the tumor and patient survival. We performed immunohistochemical staining of ezrin, ERK, STAT3, and AKT in tumors from patients with tongue carcinoma in situ (CIS, n = 17) and tongue squamous cell carcinoma (SCC, n = 46). Statistical differences between the SCC versus the CIS cohorts were estimated by calculations of bivariate odds ratios of low versus high expression of the proteins. Fisher's exact tests were used to appraise interassociations between the proteins, as well as expression levels versus patient and tumor characteristics. Survival based on Kaplan-Meier statistics in combination log-rank tests were used to address potential effects of the patient and tumor characteristics versus 5-year survival rate. The relative high: low expression of all four proteins in the two cohorts differed, and particularly ERK was markedly overexpressed in the SCC versus the CIS cohort (odds ratio = 45.3, p < .01). The relative high: low expression each protein versus patient and tumor characteristics; showed associations between AKT expression and T stage (p = .002) plus node metastases (p = .12), and between ERK expression and drinking (p = .01) and smoking history (p = .01). There was no significant difference observed between ERK and the three other molecules, nor any significant difference between the degree of expression of each protein and the 5-year disease-specific survival rate. Ezrin, ERK, STAT3, and AKT appear to be involved in the progress from carcinoma in situ in the tongue into squamous cell carcinoma. ERK in particular is overexpressed, suggesting that ERK may be a novel therapeutic target for preventing tongue cancer.

Sections du résumé

BACKGROUND
Ezrin, ERK, STAT3, and AKT are proteins that are overexpressed in various types of cancer, although their expressions in tongue cancer has received less focus. This study aimed to address associations between the expression levels of these proteins and with characteristics of the tumor and patient survival.
METHODS
We performed immunohistochemical staining of ezrin, ERK, STAT3, and AKT in tumors from patients with tongue carcinoma in situ (CIS, n = 17) and tongue squamous cell carcinoma (SCC, n = 46). Statistical differences between the SCC versus the CIS cohorts were estimated by calculations of bivariate odds ratios of low versus high expression of the proteins. Fisher's exact tests were used to appraise interassociations between the proteins, as well as expression levels versus patient and tumor characteristics. Survival based on Kaplan-Meier statistics in combination log-rank tests were used to address potential effects of the patient and tumor characteristics versus 5-year survival rate.
RESULTS
The relative high: low expression of all four proteins in the two cohorts differed, and particularly ERK was markedly overexpressed in the SCC versus the CIS cohort (odds ratio = 45.3, p < .01). The relative high: low expression each protein versus patient and tumor characteristics; showed associations between AKT expression and T stage (p = .002) plus node metastases (p = .12), and between ERK expression and drinking (p = .01) and smoking history (p = .01). There was no significant difference observed between ERK and the three other molecules, nor any significant difference between the degree of expression of each protein and the 5-year disease-specific survival rate.
CONCLUSION
Ezrin, ERK, STAT3, and AKT appear to be involved in the progress from carcinoma in situ in the tongue into squamous cell carcinoma. ERK in particular is overexpressed, suggesting that ERK may be a novel therapeutic target for preventing tongue cancer.

Identifiants

pubmed: 32281236
doi: 10.1002/cre2.293
pmc: PMC7453773
doi:

Substances chimiques

Biomarkers, Tumor 0
Cytoskeletal Proteins 0
STAT3 Transcription Factor 0
STAT3 protein, human 0
ezrin 0
Proto-Oncogene Proteins c-akt EC 2.7.11.1
MAPK1 protein, human EC 2.7.11.24
MAPK3 protein, human EC 2.7.11.24
Mitogen-Activated Protein Kinase 1 EC 2.7.11.24
Mitogen-Activated Protein Kinase 3 EC 2.7.11.24

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

420-427

Informations de copyright

© 2020 The Authors. Clinical and Experimental Dental Research published by John Wiley & Sons Ltd.

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Auteurs

Masaharu Noi (M)

Department of Oral and Maxillofacial Surgery, Shiga University of Medical Science, Ōtsu, Japan.
Division of Molecular Diagnostic Pathology, Department of Pathology, Shiga University of Medical Science, Ōtsu, Japan.

Ken-Ichi Mukaisho (KI)

Division of Molecular Diagnostic Pathology, Department of Pathology, Shiga University of Medical Science, Ōtsu, Japan.

Shoko Murakami (S)

Department of Oral and Maxillofacial Surgery, Shiga University of Medical Science, Ōtsu, Japan.
Division of Molecular Diagnostic Pathology, Department of Pathology, Shiga University of Medical Science, Ōtsu, Japan.

Shinya Koshinuma (S)

Department of Oral and Maxillofacial Surgery, Shiga University of Medical Science, Ōtsu, Japan.

Yoshisato Machida (Y)

Department of Oral and Maxillofacial Surgery, Shiga University of Medical Science, Ōtsu, Japan.

Masashi Yamori (M)

Department of Oral and Maxillofacial Surgery, Shiga University of Medical Science, Ōtsu, Japan.

Takahisa Nakayama (T)

Division of Molecular Diagnostic Pathology, Department of Pathology, Shiga University of Medical Science, Ōtsu, Japan.

Takao Ogawa (T)

Department of Otorhinolaryngology, Shiga University of Medical Science, Ōtsu, Japan.

Yusuke Nakata (Y)

Department of Otorhinolaryngology, Shiga University of Medical Science, Ōtsu, Japan.

Takeshi Shimizu (T)

Department of Otorhinolaryngology, Shiga University of Medical Science, Ōtsu, Japan.

Gaku Yamamoto (G)

Department of Oral and Maxillofacial Surgery, Shiga University of Medical Science, Ōtsu, Japan.

Hiroyuki Sugihara (H)

Division of Molecular Diagnostic Pathology, Department of Pathology, Shiga University of Medical Science, Ōtsu, Japan.

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Classifications MeSH