Cryptotanshinone reverses the epithelial-mesenchymal transformation process and attenuates bleomycin-induced pulmonary fibrosis.
Animals
Antibiotics, Antineoplastic
/ adverse effects
Bleomycin
/ adverse effects
Disease Models, Animal
Drugs, Chinese Herbal
/ pharmacology
Epithelial-Mesenchymal Transition
/ drug effects
Humans
Male
Mice
Mice, Inbred C57BL
Phenanthrenes
/ pharmacology
Pulmonary Fibrosis
/ chemically induced
Rats
EMT
attenuate
cryptotanshinone
pulmonary fibrosis
Journal
Phytotherapy research : PTR
ISSN: 1099-1573
Titre abrégé: Phytother Res
Pays: England
ID NLM: 8904486
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
30
12
2019
revised:
13
03
2020
accepted:
25
03
2020
pubmed:
14
4
2020
medline:
1
12
2020
entrez:
14
4
2020
Statut:
ppublish
Résumé
Idiopathic pulmonary fibrosis (IPF) is a fibrotic interstitial pneumonia that causes pulmonary tissue damage and functional impairment. To investigate the effects of cryptotanshinone on pulmonary fibrosis, the expression of NIH/3T3, HPF, and rat primary pulmonary fibroblasts was measured and found to be inhibited by CPT in a time- and concentration-dependent manner, and the upregulation of α-SMA expression in NIH/3T3 and HPF cells, which had been stimulated by TGFβ-1, was decreased after CPT administration. We observed that CPT could reverse the increase in α-SMA expression and vimentin and the decrease in E-cad expression in A549 cells, which had been induced by 5 ng/mL TGFβ-1, indicating that CPT has inhibitory effects in the EMT process. A BLM-induced pulmonary fibrosis model was established in C57BL/6 mice. The lung coefficient and hydroxyproline content increased significantly in the BLM-induced group and were decreased in the CPT-treated group. The expression levels of collagen-I and α-SMA and the phosphorylation level of Stat3 were significantly increased, and CPT treatment decreased these levels. Furthermore, the results from the flow cytometry analysis indicated that, in lung tissues, the frequencies of MDSCs, macrophages, DCs and T cells were considerably increased in the BLM-induced group, while CPT treatment reduced these immunocyte populations.
Substances chimiques
Antibiotics, Antineoplastic
0
Drugs, Chinese Herbal
0
Phenanthrenes
0
Bleomycin
11056-06-7
cryptotanshinone
5E9SXT166N
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2685-2696Subventions
Organisme : National Natural Science Foundation of China
ID : 81500054
Organisme : Sichuan University
ID : 2018YJSY110
Informations de copyright
© 2020 John Wiley & Sons, Ltd.
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