Malaria parasitemia among blood donors in Uganda.
Adolescent
Adult
Asymptomatic Infections
/ epidemiology
Blood Donors
/ statistics & numerical data
Blood Transfusion
/ statistics & numerical data
Cross-Sectional Studies
Female
Humans
Malaria
/ blood
Malaria, Falciparum
/ blood
Male
Middle Aged
Parasitemia
/ blood
Plasmodium falciparum
/ growth & development
Plasmodium malariae
/ growth & development
Plasmodium ovale
/ growth & development
Prevalence
Uganda
/ epidemiology
Young Adult
Journal
Transfusion
ISSN: 1537-2995
Titre abrégé: Transfusion
Pays: United States
ID NLM: 0417360
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
24
10
2019
revised:
17
02
2020
accepted:
17
02
2020
pubmed:
14
4
2020
medline:
9
9
2020
entrez:
14
4
2020
Statut:
ppublish
Résumé
Malaria remains a leading transfusion associated infectious risk in endemic areas. However, the prevalence of malaria parasitemia has not been well characterized in blood donor populations. This study sought to determine the prevalence of Plasmodium in red blood cell (RBC) and whole blood (WB) units after the rainy season in Uganda. Between May and July 2018, blood was collected from the sample diversion pouch of 1000 WB donors in Kampala and Jinja, Uganda. The RBC pellet from ethylenediamine tetraacetic acid (EDTA) anticoagulated blood was stored at -80°C until testing. DNA was extracted and nested PCR was used to screen samples at the genus level for Plasmodium, with positive samples further tested for species identification. Malaria parasitemia among asymptomatic, eligible blood donors in two regions of Uganda was 15.4%; 87.7% (135/154) of infections were with P. falciparum, while P. malariae and P. ovale were also detected. There were 4.3% of blood donors who had mixed infection with multiple species. Older donors (>30 years vs. 17-19 years; aPR = 0.31 [95% CI = 0.17-0.58]), females (aPR = 0.60 [95% CI = 0.42-0.87]), repeat donors (aPR = 0.44 [95% CI = 0.27-0.72]) and those donating near the capital city of Kampala versus rural Jinja region (aPR = 0.49 [95% CI = 0.34-0.69]) had a lower prevalence of malaria parasitemia. A high proportion of asymptomatic blood donors residing in a malaria endemic region demonstrate evidence of parasitemia at time of donation. Further research is needed to quantify the risk and associated burden of transfusion-transmitted malaria (TTM) in order to inform strategies to prevent TTM.
Sections du résumé
BACKGROUND
Malaria remains a leading transfusion associated infectious risk in endemic areas. However, the prevalence of malaria parasitemia has not been well characterized in blood donor populations. This study sought to determine the prevalence of Plasmodium in red blood cell (RBC) and whole blood (WB) units after the rainy season in Uganda.
METHODS AND MATERIALS
Between May and July 2018, blood was collected from the sample diversion pouch of 1000 WB donors in Kampala and Jinja, Uganda. The RBC pellet from ethylenediamine tetraacetic acid (EDTA) anticoagulated blood was stored at -80°C until testing. DNA was extracted and nested PCR was used to screen samples at the genus level for Plasmodium, with positive samples further tested for species identification.
RESULTS
Malaria parasitemia among asymptomatic, eligible blood donors in two regions of Uganda was 15.4%; 87.7% (135/154) of infections were with P. falciparum, while P. malariae and P. ovale were also detected. There were 4.3% of blood donors who had mixed infection with multiple species. Older donors (>30 years vs. 17-19 years; aPR = 0.31 [95% CI = 0.17-0.58]), females (aPR = 0.60 [95% CI = 0.42-0.87]), repeat donors (aPR = 0.44 [95% CI = 0.27-0.72]) and those donating near the capital city of Kampala versus rural Jinja region (aPR = 0.49 [95% CI = 0.34-0.69]) had a lower prevalence of malaria parasitemia.
CONCLUSIONS
A high proportion of asymptomatic blood donors residing in a malaria endemic region demonstrate evidence of parasitemia at time of donation. Further research is needed to quantify the risk and associated burden of transfusion-transmitted malaria (TTM) in order to inform strategies to prevent TTM.
Identifiants
pubmed: 32282944
doi: 10.1111/trf.15775
pmc: PMC7908807
mid: NIHMS1665312
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
955-964Subventions
Organisme : National Institute of Allergy and Infectious Diseases
ID : T32AI102623
Pays : International
Organisme : National Institute of Allergy and Infectious Diseases
ID : R01AI128779
Pays : International
Organisme : NIAID NIH HHS
ID : R01 AI120938
Pays : United States
Organisme : U.S. Department of Defense Peer Reviewed Medical Research Program
ID : W81XWH1810742
Pays : International
Organisme : NIAID NIH HHS
ID : R01 AI128779
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI102623
Pays : United States
Organisme : NHLBI NIH HHS
ID : K23 HL151826
Pays : United States
Informations de copyright
© 2020 AABB.
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