Dexmedetomidine alleviated neuropathic pain in dorsal root ganglion neurons by inhibition of anaerobic glycolysis activity and enhancement of ROS tolerance.
Acetylcysteine
/ pharmacology
Anaerobiosis
/ drug effects
Animals
Apoptosis
/ drug effects
Cells, Cultured
Dexmedetomidine
/ pharmacology
Disease Models, Animal
Ganglia, Spinal
/ cytology
Glucose
/ analysis
Glycolysis
/ drug effects
Humans
Hydrogen Peroxide
/ toxicity
Neuralgia
/ chemically induced
Neurons
/ drug effects
Primary Cell Culture
Rats
Reactive Oxygen Species
/ antagonists & inhibitors
anaerobic glycolysis
apoptosis
dexmedetomidine
dorsal root ganglion neurons
neuropathic pain
reactive oxygen species
Journal
Bioscience reports
ISSN: 1573-4935
Titre abrégé: Biosci Rep
Pays: England
ID NLM: 8102797
Informations de publication
Date de publication:
29 05 2020
29 05 2020
Historique:
received:
13
06
2019
revised:
23
02
2020
accepted:
08
04
2020
pubmed:
15
4
2020
medline:
30
3
2021
entrez:
15
4
2020
Statut:
ppublish
Résumé
Neuropathic pain is a kind of chronic pain that is triggered or caused primarily by damage to the nervous system and neurological dysfunction. It's known that dexmedetomidine is a new type of highly selective alpha2-adrenoceptor agonist with sedation, anti-anxiety, analgesic and other effects. However, the function and mechanism of dexmedetomidine on neuropathic pain are not clear. Rat DRG neurons were isolated and identified using immunofluorescence assay. Following treatment with H2O2, dexmedetomidine or ROS inhibitor (NAC), the apoptosis and ROS levels were examined by flow cytometery; apoptosis- and anaerobic glycolysis-related proteins were determined by Western blot assay; glucose consumption, pyruvic acid, lactic acid and ATP/ADP ratios were also measured. The results revealed that dexmedetomidine inhibited H2O2-induced apoptosis and reactive oxygen species (ROS) in rat DRG neurons and in addition, dexmedetomidine down-regulated the expression levels of anaerobic glycolysis-related proteins, significantly reduced glucose, pyruvic acid and lactic acid levels. It also increased the ATP/ADP ratio in H2O2-treated rat dorsal root ganglion (DRG) neurons. Moreover, we also demonstrated that ROS inhibitor (NAC) also inhibited H2O2-induced apoptosis and anaerobic glycolysis in rat DRG neurons. In conclusion, dexmedetomidine suppressed H2O2-induced apoptosis and anaerobic glycolysis activity by inhibiting ROS, in rat DRG neurons. Therefore, dexmedetomidine might play a pivotal role in neuropathic pain by the inhibition of ROS.
Identifiants
pubmed: 32285913
pii: 222638
doi: 10.1042/BSR20191994
pmc: PMC7201561
pii:
doi:
Substances chimiques
Reactive Oxygen Species
0
Dexmedetomidine
67VB76HONO
Hydrogen Peroxide
BBX060AN9V
Glucose
IY9XDZ35W2
Acetylcysteine
WYQ7N0BPYC
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2020 The Author(s).
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