High Expression of the Sd
gene expression
glycosylation
glycosyltransferases
microarray analysis
stemness
sugar antigens
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
11 04 2020
11 04 2020
Historique:
received:
25
02
2020
revised:
06
04
2020
accepted:
07
04
2020
entrez:
16
4
2020
pubmed:
16
4
2020
medline:
16
3
2021
Statut:
epublish
Résumé
The carbohydrate antigen Sd Correlations of B4GALNT2 mRNA with clinical data were obtained from The Cancer Genome Atlas (TCGA) database; the phenotypic and transcriptomic changes induced by B4GALNT2 were studied in LS174T cells transfected with B4GALNT2 cDNA. TCGA data indicate that patients with high B4GALNT2 expression in cancer tissues display longer survival than non-expressers. In LS174T cells, expression of B4GALNT2 did not affect the ability to heal a scratch wound or to form colonies in standard growth conditions but markedly reduced the growth in soft agar, the tridimensional (3D) growth as spheroids, and the number of cancer stem cells, indicating a specific effect of B4GALNT2 on the growth in poor adherence and stemness. On the transcriptome, B4GALNT2 induced the down-regulation of the stemness-associated gene The level of B4GALNT2 can be proposed as a marker to identify higher- and lower-risk colorectal cancer patients.
Sections du résumé
BACKGROUND
The carbohydrate antigen Sd
METHODS
Correlations of B4GALNT2 mRNA with clinical data were obtained from The Cancer Genome Atlas (TCGA) database; the phenotypic and transcriptomic changes induced by B4GALNT2 were studied in LS174T cells transfected with B4GALNT2 cDNA.
RESULTS
TCGA data indicate that patients with high B4GALNT2 expression in cancer tissues display longer survival than non-expressers. In LS174T cells, expression of B4GALNT2 did not affect the ability to heal a scratch wound or to form colonies in standard growth conditions but markedly reduced the growth in soft agar, the tridimensional (3D) growth as spheroids, and the number of cancer stem cells, indicating a specific effect of B4GALNT2 on the growth in poor adherence and stemness. On the transcriptome, B4GALNT2 induced the down-regulation of the stemness-associated gene
CONCLUSIONS
The level of B4GALNT2 can be proposed as a marker to identify higher- and lower-risk colorectal cancer patients.
Identifiants
pubmed: 32290493
pii: cells9040948
doi: 10.3390/cells9040948
pmc: PMC7226961
pii:
doi:
Substances chimiques
Oligosaccharides
0
Sd(a) determinant
0
N-Acetylgalactosaminyltransferases
EC 2.4.1.-
beta-1,4-N-acetyl-galactosaminyl transferase 2
EC 2.4.1.165
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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