NGF from pancreatic stellate cells induces pancreatic cancer proliferation and invasion by PI3K/AKT/GSK signal pathway.
Cell Line, Tumor
Cell Proliferation
Epithelial-Mesenchymal Transition
/ genetics
Gene Expression Regulation, Neoplastic
Glycogen Synthase Kinase 3 beta
/ metabolism
Humans
Neoplasm Invasiveness
Nerve Growth Factor
/ metabolism
Pancreas
/ pathology
Pancreatic Neoplasms
/ genetics
Pancreatic Stellate Cells
/ metabolism
Phosphatidylinositol 3-Kinases
/ metabolism
Proto-Oncogene Proteins c-akt
/ metabolism
RNA, Small Interfering
/ metabolism
Receptor, trkA
/ metabolism
Signal Transduction
NGF
TrkA
epithelial-mesenchymal transition
pancreatic cancer
proliferation
Journal
Journal of cellular and molecular medicine
ISSN: 1582-4934
Titre abrégé: J Cell Mol Med
Pays: England
ID NLM: 101083777
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
30
01
2020
revised:
12
03
2020
accepted:
18
03
2020
pubmed:
16
4
2020
medline:
1
5
2021
entrez:
16
4
2020
Statut:
ppublish
Résumé
Pancreatic cancer (PC) is a continuously high lethal disease, and the tumour microenvironment plays a pivotal role during PC progression. Herein, we focus on that the Nerve growth factor (NGF)/Tropomyosin-related kinase A (TrkA), in pancreatic stellate cells-pancreatic cancer cells (PSCs-PC cells) co-culture system, influences PC proliferation and invasion. The model of PC cells and PSCs was directly co-cultured in a no-touch manner, using the Transwell as the co-culture system. NGF and TrkA expression was measured in cultured system by real-time PCR, immunofluorescence, Western blotting analysis or ELISA. Small interfering RNA transfection was used to regulate the expression of TrkA in PC cells. The promotion of cancer invasion was investigated using Matrigel Transwell assay. In our study, NGF/TrkA is overexpressed in PSCs-PC cells co-culture system and promotes the invasion and proliferation of PC cells. And the epithelial-mesenchymal transition-related genes are influenced by si-TrkA. What's more, NGF/TrkA regulates the PC cell proliferation and invasion via activation of PI3K/AKT/GSK signalling. The present study demonstrated NGF/TrkA promoted the PC cell proliferation and invasion in the co-culture system by the activation of the PI3K/AKT/GSK signal cascade, providing a potential therapeutic target for PC patients.
Identifiants
pubmed: 32294802
doi: 10.1111/jcmm.15265
pmc: PMC7214160
doi:
Substances chimiques
RNA, Small Interfering
0
Nerve Growth Factor
9061-61-4
Receptor, trkA
EC 2.7.10.1
Glycogen Synthase Kinase 3 beta
EC 2.7.11.1
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5901-5910Informations de copyright
© 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
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