Minimization of artifact protein aggregation using tetradecyl sulfate and hexadecyl sulfate in capillary gel electrophoresis under reducing conditions.
Capillary gel electrophoresis
Detergent affinity
Hydrophobic detergent
Method robustness
Protein therapeutic
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
26
11
2019
revised:
31
03
2020
accepted:
01
04
2020
pubmed:
17
4
2020
medline:
23
3
2021
entrez:
17
4
2020
Statut:
ppublish
Résumé
In the biopharmaceutical industry, CE-SDS assesses the purity, heterogeneity, and stability of therapeutic proteins. However, for mAb-1 and mAb-2, typical CE-SDS under reducing conditions produced atypical protein peak profiles, which led to biased purity results, thus were not acceptable for biologics manufacturing. This bias was caused by the formation of method-induced higher molecular weight artifacts, the levels of which correlated with protein concentration. Here we show that adding sodium tetradecyl and hexadecyl sulfates to the sample and the sieving gel buffer solutions was required to prevent formation of aggregate artifacts and to maintain detergent:protein uniformity, suggesting their importance during the sample preparation steps of heat denaturation and subsequent cooling as well as during capillary migration. For these proteins, we show that this uniformity was likely due to the ability of these detergents to bind proteins with markedly higher affinities compared to SDS. "CE-SC
Identifiants
pubmed: 32297333
doi: 10.1002/elps.201900435
doi:
Substances chimiques
Antibodies, Monoclonal
0
Detergents
0
Protein Aggregates
0
Proteins
0
Sodium Tetradecyl Sulfate
Q1SUG5KBD6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1245-1252Informations de copyright
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Références
Reichert, J. M., Curr. Pharm. Biotechnol. 2008, 9, 423-430.
Ecker, D. M., Jones, S. D., Levine, H. L., mAbs 2015, 7, 9-14.
Weiner, L. M., Surana, R., Wang, S., Nat. Rev. Immunol. 2010, 10, 317-327.
Creamer, J. S., Oborny, N. J., Lunte, S. M., Anal. Methods 2014, 6, 5427-5449.
Manning, M. C., Chou, D. K., Murphy, B. M., Payne, R. W., Katayama, D. S., Pharm. Res. 2010, 27, 544-575.
Fekete, S., Gassner, A., Rudaz, S., Schappler, J., Gulillarme, D., TrAC Trends Anal. Chem. 2013, 42, 74-83.
Shapiro, A., Vinuela, E., Maizel, J., Biochem. Biophys. Res. Commun. 1967, 28, 815-820.
Turner, A., Yandrofski, K., Telikepalli, S., King, J., Herckert, A., Filliben, J., Ripple, D., Schiel, J. E., Anal. Bioanal. Chem. 2018, 410, 2095-2110.
Ganzler, K., Greve, K., Cohen, A., Karger, B., Guttman, A., Cooke, N., Anal. Chem. 1992, 64, 2665-2671.
Cohen, A., Karger, B., J. Chromatogr. A 1987, 397, 409-417.
Zhu, Z., Lu, J. J., Liu, S., Anal. Chim. Acta 2012, 709, 21-31.
Rustandi, R. R., Washabaugh, M. W., Wang, Y., Electrophoresis 2008, 29, 3612-3620.
Hunt, G., Nashabeh, W., Anal. Chem. 1999, 71, 2390-2397.
Reynolds, J. A., Tanford, C., J. Biol. Chem. 1970, 245, 5161-5165.
Otzen, D. E., Curr. Opin. Colloid Interface Sci. 2015, 20, 161-169.
Manning, M., Colon, W., Biochemistry 2004, 43, 11248-11254.
Pitt-Rivers, R., Impiombato, F., Biochem. J. 1968, 109, 825-830.
Guttman, A., Nolan, J., Anal. Biochem. 1994, 221, 285-289.
Beckman, J., Song, Y., Gu, Y., Voronov, S., Chennamsetty, N., Krystek, S., Mussa, N., Li, Z. J., Anal. Chem. 2018, 90, 2542-2547.
Shaw, B. F., Schneider, G. F., Arthanari, H., Narovlyansky, M., Moustakas, D., Durazo, A., Wagner, G., Whitesides, G. M., J. Am. Chem. Soc. 2011, 133, 17681-17695.
Shaw, B. F., Schneider, G. F., Whitesides, G. M., J. Am. Chem. Soc. 2012, 134, 18739-18745.
Bean, S., Lookhart, G., J. Agric. Food Chem. 1999, 47, 4246-4255.
Beckman, J. W., Guan, Q., Patent application number PCT/US2018/049992, 2018.
Liu, Y., Reddy, M., Ratnayake, C., Koh, E., Patent application number 20090314638, 2009.
Tous, G. I., Wei, Z., Feng, J., Bilbulian, S., Bowen, S., Smith, J., Strouse, R., McGeehan, P., Casas-Finet, J., Schenerman, M. A., Anal. Chem. 2005, 77, 2675-2682.
Tsai, A. M., Vvan Zarten, J. H., Betenbaugh, M., J. Biotechnol. Bioeng. 1998, 59, 281-285.
Zhang, J., Burman, S., Gunturi, S., Foley, J. P., J. Pharm. Biomed. Anal. 2010, 53, 1236-1243.
Salas-Solano, O., Tomlinson, B., Du, S., Parker, M., Strahan, A., Ma, S., Anal. Chem. 2006, 78, 6583-6594.
Nelson, C. A., J. Biol. Chem. 1971, 246, 3895-3901.
Otzen, D., Christiansen, L., Schulein, M., Protein Sci. 1999, 8, 1878-1887.
Ospinal-Jimenez, M., Pozzo, D. C., Langmuir 2011, 27, 928-935.