Two fatty acid-binding proteins expressed in the intestine interact differently with endocannabinoids.
NMR
chemical shift perturbation
endocannabinoid ligand binding affinity
endocannabinoids
fatty acid ethanolamide
fatty acid-binding protein
fatty acids
fluorescence
intestinal FABP
liver-type FABP
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
06
01
2020
revised:
08
04
2020
accepted:
12
04
2020
pubmed:
17
4
2020
medline:
16
1
2021
entrez:
17
4
2020
Statut:
ppublish
Résumé
Two different members of the fatty acid-binding protein (FABP) family are found in enterocyte cells of the gastrointestinal system, namely liver-type and intestinal fatty acid-binding proteins (LFABP and IFABP, also called FABP1 and FABP2, respectively). Striking phenotypic differences have been observed in knockout mice for either protein, for example, high fat-fed IFABP-null mice remained lean, whereas LFABP-null mice were obese, correlating with differences in food intake. This finding prompted us to investigate the role each protein plays in directing the specificity of binding to ligands involved in appetite regulation, such as fatty acid ethanolamides and related endocannabinoids. We determined the binding affinities for nine structurally related ligands using a fluorescence competition assay, revealing tighter binding to IFABP than LFABP for all ligands tested. We found that the head group of the ligand had more impact on binding affinity than the alkyl chain, with the strongest binding observed for the carboxyl group, followed by the amide, and then the glycerol ester. These trends were confirmed using two-dimensional
Identifiants
pubmed: 32298508
doi: 10.1002/pro.3875
pmc: PMC7314394
doi:
Substances chimiques
Endocannabinoids
0
Fabp1 protein, mouse
0
Fabp2 protein, mouse
0
Fatty Acid-Binding Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1606-1617Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK038389
Pays : United States
Organisme : NIH HHS
ID : CO6RR015495
Pays : United States
Organisme : NIMHD NIH HHS
ID : G12 MD007603
Pays : United States
Organisme : NIMHD NIH HHS
ID : 5G12-MD007603-30
Pays : United States
Organisme : NCRR NIH HHS
ID : C06 RR015495
Pays : United States
Organisme : NIH HHS
ID : 5R01-DK038389-31
Pays : United States
Informations de copyright
© 2020 The Protein Society.
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