Coating M-CSF on plastic surface results in the generation of increased numbers of macrophages in vitro.
Bone marrow
Innate immunity
Macrophages
Journal
Journal of immunological methods
ISSN: 1872-7905
Titre abrégé: J Immunol Methods
Pays: Netherlands
ID NLM: 1305440
Informations de publication
Date de publication:
Historique:
received:
16
01
2020
revised:
29
03
2020
accepted:
31
03
2020
pubmed:
19
4
2020
medline:
20
1
2021
entrez:
19
4
2020
Statut:
ppublish
Résumé
Macrophages are one of the important cell types in the innate immune system that are present in various anatomical regions of the body and promote early control of pathogens. The relative proportion of macrophages in various lymphoid and non-lymphoid regions is small, and as such it is tedious to purify these cells to homogeneity. Culture of bone marrow precursors with macrophage colony-stimulating factor (M-CSF) results in their differentiation to macrophages, however this procedure results in low numbers of differentiated macrophages. Herein we reveal a new approach of generating increased numbers of differentiated macrophages from bone marrow precursors. We show that M-CSF delivered in a plate-bound form results in the differentiation of significantly more macrophages in comparison to soluble M-CSF. Furthermore, the macrophages differentiated with plate-bound M-CSF display increased metabolic activity and cell death following infection with pathogens.
Identifiants
pubmed: 32304707
pii: S0022-1759(20)30067-3
doi: 10.1016/j.jim.2020.112788
pii:
doi:
Substances chimiques
Plastics
0
Macrophage Colony-Stimulating Factor
81627-83-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
112788Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.