Comparison of metal-bound and unbound structures of aminopeptidase B proteins from Escherichia coli and Yersinia pestis.


Journal

Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750

Informations de publication

Date de publication:
07 2020
Historique:
received: 09 02 2020
revised: 14 04 2020
accepted: 14 04 2020
pubmed: 20 4 2020
medline: 16 1 2021
entrez: 20 4 2020
Statut: ppublish

Résumé

Protein degradation by aminopeptidases is involved in bacterial responses to stress. Escherichia coli produces two metal-dependent M17 family leucine aminopeptidases (LAPs), aminopeptidase A (PepA) and aminopeptidase B (PepB). Several structures have been solved for PepA as well as other bacterial M17 peptidases. Herein, we report the first structures of a PepB M17 peptidase. The E. coli PepB protein structure was determined at a resolution of 2.05 and 2.6 Å. One structure has both Zn

Identifiants

pubmed: 32306515
doi: 10.1002/pro.3876
pmc: PMC7314395
doi:

Substances chimiques

Escherichia coli Proteins 0
Manganese 42Z2K6ZL8P
Aminopeptidases EC 3.4.11.-
Zinc J41CSQ7QDS

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1618-1628

Subventions

Organisme : NIAID NIH HHS
ID : U01 AI124316
Pays : United States
Organisme : NIAID NIH HHS
ID : U01AI124316
Pays : United States
Organisme : NIAID NIH HHS
ID : HHSN272201700060C
Pays : United States
Organisme : NIAID NIH HHS
ID : HHSN272201200026C
Pays : United States

Informations de copyright

© 2020 The Protein Society.

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Auteurs

George Minasov (G)

Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
Center for Structural Genomics of Infectious Diseases, Northwestern University, Chicago, Illinois, USA.

Matthew R Lam (MR)

Department of Molecular Biosciences, Weinberg School of Arts and Sciences, Northwestern University, Evanston, Illinois, USA.

Monica Rosas-Lemus (M)

Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
Center for Structural Genomics of Infectious Diseases, Northwestern University, Chicago, Illinois, USA.

Joanna Sławek (J)

Center for Structural Genomics of Infectious Diseases, Northwestern University, Chicago, Illinois, USA.
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia, USA.

Magdalena Woinska (M)

Center for Structural Genomics of Infectious Diseases, Northwestern University, Chicago, Illinois, USA.
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia, USA.

Ivan G Shabalin (IG)

Center for Structural Genomics of Infectious Diseases, Northwestern University, Chicago, Illinois, USA.
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia, USA.

Ludmilla Shuvalova (L)

Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
Center for Structural Genomics of Infectious Diseases, Northwestern University, Chicago, Illinois, USA.

Bernhard Ø Palsson (BØ)

Department of Bioengineering and Pediatrics, University of California, San Diego, California, USA.

Adam Godzik (A)

Center for Structural Genomics of Infectious Diseases, Northwestern University, Chicago, Illinois, USA.
Department of Biomedical Sciences, University of California, Riverside School of Medicine, Riverside, California, USA.

Wladek Minor (W)

Center for Structural Genomics of Infectious Diseases, Northwestern University, Chicago, Illinois, USA.
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia, USA.

Karla J F Satchell (KJF)

Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
Center for Structural Genomics of Infectious Diseases, Northwestern University, Chicago, Illinois, USA.

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