A benchmark data set for the mechanical properties of double-stranded DNA and RNA under torsional constraint.
DNA
Linking number
Magnetic tweezers
Mechanics
RNA
Single-molecule
Torsional stiffness
Journal
Data in brief
ISSN: 2352-3409
Titre abrégé: Data Brief
Pays: Netherlands
ID NLM: 101654995
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
12
02
2020
revised:
02
03
2020
accepted:
03
03
2020
entrez:
21
4
2020
pubmed:
21
4
2020
medline:
21
4
2020
Statut:
epublish
Résumé
Nucleic acids are central to the storage and transmission of genetic information and play essential roles in many cellular processes. Quantitative understanding and modeling of their functions and properties requires quantitative experimental characterization. We use magnetic tweezers (MT) to apply precisely calibrated stretching forces and linking number changes to DNA and RNA molecules tethered between a surface and superparamagnetic beads. Magnetic torque tweezers (MTT) allow to control the linking number of double-stranded DNA or RNA tethers, while directly measuring molecular torque by monitoring changes in the equilibrium rotation angle upon over- or underwinding of the helical molecules. Here, we provide a comprehensive data set of double-stranded DNA and RNA under controlled stretching as a function of the linking number. We present data for extension and torque as a function of linking number in equilibrium. We report data for the critical torque of buckling and of the torsional stiffness of DNA and RNA as a function of applied force. Finally, we provide dynamic data for the hopping behavior at the DNA buckling point.
Identifiants
pubmed: 32309523
doi: 10.1016/j.dib.2020.105404
pii: S2352-3409(20)30298-5
pii: 105404
pmc: PMC7154992
doi:
Types de publication
Journal Article
Langues
eng
Pagination
105404Informations de copyright
© 2020 The Author(s).
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