Cytokines, Angiogenesis, and Extracellular Matrix Degradation are Augmented by Oxidative Stress in Endometriosis.
Adult
Body Mass Index
Case-Control Studies
Cyclooxygenase 2
/ blood
Cytokines
/ blood
Discriminant Analysis
Endometriosis
/ metabolism
Estrogens
/ blood
Extracellular Matrix
/ metabolism
Female
Humans
Interleukin-10
/ blood
Matrix Metalloproteinase 2
/ blood
Multivariate Analysis
NF-kappa B
/ metabolism
Oxidative Stress
Principal Component Analysis
Retrospective Studies
Tissue Inhibitor of Metalloproteinase-1
/ blood
Vascular Endothelial Growth Factor A
/ blood
Cytokines
Endometriosis
Extracellular matrix
Oxidative stress (OS)
Journal
Annals of laboratory medicine
ISSN: 2234-3814
Titre abrégé: Ann Lab Med
Pays: Korea (South)
ID NLM: 101571172
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
17
09
2019
revised:
26
01
2020
accepted:
01
04
2020
entrez:
21
4
2020
pubmed:
21
4
2020
medline:
7
1
2021
Statut:
ppublish
Résumé
The effect of the interplay among inflammation, angiogenesis, extracellular matrix (ECM) degradation, and oxidative stress (OS) on the pathogenesis of endometriosis remains unclear. Previously, we demonstrated the role of OS in endometriosis. Here, we performed a comprehensive investigation of several molecules involved in inflammation, angiogenesis, and ECM degradation in women with endometriosis to study their interplay with OS. Blood samples were collected from women with endometriosis (N=80), as well as from women with tubal factor infertility as controls (N=80). Interleukin (IL)-1β, tumor necrosis factor-alpha, interferon-gamma, transforming growth factor-beta, IL-4, -10, -2, -6, -8, vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2, -9, tissue inhibitor of metalloproteinases (TIMP)-1, -2, and cyclooxygenase (COX)-2 levels in serum samples were measured using an ELISA. Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in peripheral blood mononuclear cells was measured using flow cytometry. Cytokines, VEGF, MMPs, and COX-2 were significantly higher and TIMPs were significantly lower in patients with endometriosis. Multivariate statistical analysis indicated that IL-10 was the most significant variable capable of discriminating endometriosis samples from controls. Deregulation of NF-κB activation by OS affects the expression of various cytokines in endometriosis. Elevated cytokine levels further up-regulate IL-10, which subsequently activates the MMPs, leading to excessive ECM degradation and angiogenesis. Moreover, IL-10 emerged as the most important molecule involved in the pathogenesis of endometriosis. Measurement of these molecules may help in better management of the patients with endometriosis.
Sections du résumé
BACKGROUND
The effect of the interplay among inflammation, angiogenesis, extracellular matrix (ECM) degradation, and oxidative stress (OS) on the pathogenesis of endometriosis remains unclear. Previously, we demonstrated the role of OS in endometriosis. Here, we performed a comprehensive investigation of several molecules involved in inflammation, angiogenesis, and ECM degradation in women with endometriosis to study their interplay with OS.
METHODS
Blood samples were collected from women with endometriosis (N=80), as well as from women with tubal factor infertility as controls (N=80). Interleukin (IL)-1β, tumor necrosis factor-alpha, interferon-gamma, transforming growth factor-beta, IL-4, -10, -2, -6, -8, vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2, -9, tissue inhibitor of metalloproteinases (TIMP)-1, -2, and cyclooxygenase (COX)-2 levels in serum samples were measured using an ELISA. Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in peripheral blood mononuclear cells was measured using flow cytometry.
RESULTS
Cytokines, VEGF, MMPs, and COX-2 were significantly higher and TIMPs were significantly lower in patients with endometriosis. Multivariate statistical analysis indicated that IL-10 was the most significant variable capable of discriminating endometriosis samples from controls.
CONCLUSIONS
Deregulation of NF-κB activation by OS affects the expression of various cytokines in endometriosis. Elevated cytokine levels further up-regulate IL-10, which subsequently activates the MMPs, leading to excessive ECM degradation and angiogenesis. Moreover, IL-10 emerged as the most important molecule involved in the pathogenesis of endometriosis. Measurement of these molecules may help in better management of the patients with endometriosis.
Identifiants
pubmed: 32311852
doi: 10.3343/alm.2020.40.5.390
pii: alm-2020-40-5-390
pmc: PMC7169633
doi:
Substances chimiques
Cytokines
0
Estrogens
0
NF-kappa B
0
Tissue Inhibitor of Metalloproteinase-1
0
Vascular Endothelial Growth Factor A
0
Interleukin-10
130068-27-8
Cyclooxygenase 2
EC 1.14.99.1
Matrix Metalloproteinase 2
EC 3.4.24.24
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
390-397Références
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