Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017.
Adolescent
Adult
Aged
Bhutan
/ epidemiology
Child
Child, Preschool
Dengue
/ epidemiology
Dengue Virus
/ genetics
Disease Outbreaks
Female
Genotype
Humans
India
/ epidemiology
Infant
Infant, Newborn
Male
Middle Aged
Molecular Epidemiology
Phylogeny
Serogroup
Viral Envelope Proteins
/ classification
Young Adult
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
25
04
2019
accepted:
23
02
2020
entrez:
23
4
2020
pubmed:
23
4
2020
medline:
7
7
2020
Statut:
epublish
Résumé
Dengue continues to pose a significant public health problem in tropical and subtropical countries. In Bhutan, first outbreak of dengue fever (DF) was reported in 2004 in a southern border town, followed by sporadic cases over the years. In this study, we analysed DF outbreaks that occurred in 3 different places during the years 2016 and 2017. A total of 533 cases in 2016 and 163 in 2017 were suspected of having of DF, where young adults were mostly affected. A total of 240 acute serum specimens collected and analyzed for serotype by nested RT-PCR revealed predominance of serotypes 1 and 2 (DENV-1 and 2). Phylogenetic analysis using envelope gene for both the serotypes demonstrated cosmopolitan genotype which were closely related to strains from India, indicating that they were probably imported from the neighboring country over the past few years.
Identifiants
pubmed: 32320397
doi: 10.1371/journal.pntd.0008165
pii: PNTD-D-19-00558
pmc: PMC7176082
doi:
Substances chimiques
Viral Envelope Proteins
0
glycoprotein E, Flavivirus
145420-18-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0008165Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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