Amblyomma americanum serpin 41 (AAS41) inhibits inflammation by targeting chymase and chymotrypsin.
Amblyomma
/ chemistry
Animals
Anti-Inflammatory Agents
/ chemistry
Chromatography, Affinity
Chromatography, High Pressure Liquid
Chymases
/ antagonists & inhibitors
Chymotrypsin
/ antagonists & inhibitors
Disease Models, Animal
Enzyme Inhibitors
/ chemistry
Gene Expression
Glycoproteins
/ genetics
Mice
Rabbits
Rats
Recombinant Proteins
Saccharomycetales
/ genetics
Serpins
/ chemistry
Inflammation
RNAi-mediated silencing
Tick feeding physiology and tick saliva serpins
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Aug 2020
01 Aug 2020
Historique:
received:
17
02
2020
revised:
09
04
2020
accepted:
12
04
2020
pubmed:
23
4
2020
medline:
11
3
2021
entrez:
23
4
2020
Statut:
ppublish
Résumé
Ticks inject serine protease inhibitors (serpins) into their feeding sites to evade serine protease-mediated host defenses against tick-feeding. This study describes two highly identitical (97%) but functionally different Amblyomma americanum tick saliva serpins (AAS41 and 46) that are secreted at the inception of tick-feeding. We show that AAS41, which encodes a leucine at the P1 site inhibits inflammation system proteases: chymase (SI = 3.23, Ka = 5.6 ± 3.7X10
Identifiants
pubmed: 32320803
pii: S0141-8130(20)32944-5
doi: 10.1016/j.ijbiomac.2020.04.088
pii:
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Enzyme Inhibitors
0
Glycoproteins
0
Recombinant Proteins
0
Serpins
0
Chymotrypsin
EC 3.4.21.1
Chymases
EC 3.4.21.39
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1007-1021Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.