Serum amino acid profile in 51 dogs with immunosuppressant-responsive enteropathy (IRE): a pilot study on clinical aspects and outcomes.
Body condition score
CCECAI
Canine
Histidine
Tryptophan
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
22 Apr 2020
22 Apr 2020
Historique:
received:
05
11
2019
accepted:
05
04
2020
entrez:
24
4
2020
pubmed:
24
4
2020
medline:
6
1
2021
Statut:
epublish
Résumé
Lower levels of tryptophan (TRP) have been identified in people with inflammatory bowel disease and in dogs with protein-losing enteropathy (PLE). No data on serum amino acids (AAs) but some on plasma in canine immunosuppressant-responsive enteropathy (IRE) are available. The aim of this study is to compare serum AAs between healthy and IRE dogs, considering clinicopathological variables and follow-up. Twenty-six healthy control dogs (CD) and 51 IRE dogs were included. IRE was diagnosed after the exclusion of extra-intestinal diseases and food and antibiotic responsive enteropathies. The canine chronic enteropathy clinical activity index (CCECAI) was assessed at presentation and during the clinical follow-up. In CD and IRE dogs, 19 different serum AAs were measured. IRE dogs were classified into responders, partial responders and non-responders, based on CCECAI after 1 month, and divided into PLE and non-PLE, based on albumin level. IRE dogs showed lower L-Tyrosine (TYR), L-Phenylalanine (PHE) and TRP (p < 0.001) and higher L-Serine (SER), L-Glutamic acid (GLU), L-Arginine (p < 0.001), L-Threonine (p = 0.013), Proline (p = 0.044), L-Cysteine (p = 0.003), L-Valine (p = 0.018), L-Lysine (p = 0.01) and L-Isoleucine (p = 0.005) than CDs. PLE dogs showed lower L-Histidine (HIS) (p = 0.008), PHE (p = 0.005) and TRP (p = 0.005) than non-PLE dogs. In IRE dogs, median GLU was significantly lower in dogs with BCS 3/9 than BCS 5/9 category (p = 0.036). Total protein was positively correlated with PHE and TRP (both p = 0.031, r = 0.30) and albumin was positively correlated with HIS (p = 0.025, r = 0.31), PHE and TRP (both p = 0.001, r = 0.46). HIS (p = 0.041), PHE (p = 0.047) and TRP (p = 0.044) concentrations were significantly lower in non-responders than in responders and partial responders. This study may suggest further investigation on serum, HIS, PHE, TRP and TYR as markers of intestinal disease and proposed HIS, PHE and TRP as prognostic marker for response to therapy.
Sections du résumé
BACKGROUND
BACKGROUND
Lower levels of tryptophan (TRP) have been identified in people with inflammatory bowel disease and in dogs with protein-losing enteropathy (PLE). No data on serum amino acids (AAs) but some on plasma in canine immunosuppressant-responsive enteropathy (IRE) are available. The aim of this study is to compare serum AAs between healthy and IRE dogs, considering clinicopathological variables and follow-up.
RESULTS
RESULTS
Twenty-six healthy control dogs (CD) and 51 IRE dogs were included. IRE was diagnosed after the exclusion of extra-intestinal diseases and food and antibiotic responsive enteropathies. The canine chronic enteropathy clinical activity index (CCECAI) was assessed at presentation and during the clinical follow-up. In CD and IRE dogs, 19 different serum AAs were measured. IRE dogs were classified into responders, partial responders and non-responders, based on CCECAI after 1 month, and divided into PLE and non-PLE, based on albumin level. IRE dogs showed lower L-Tyrosine (TYR), L-Phenylalanine (PHE) and TRP (p < 0.001) and higher L-Serine (SER), L-Glutamic acid (GLU), L-Arginine (p < 0.001), L-Threonine (p = 0.013), Proline (p = 0.044), L-Cysteine (p = 0.003), L-Valine (p = 0.018), L-Lysine (p = 0.01) and L-Isoleucine (p = 0.005) than CDs. PLE dogs showed lower L-Histidine (HIS) (p = 0.008), PHE (p = 0.005) and TRP (p = 0.005) than non-PLE dogs. In IRE dogs, median GLU was significantly lower in dogs with BCS 3/9 than BCS 5/9 category (p = 0.036). Total protein was positively correlated with PHE and TRP (both p = 0.031, r = 0.30) and albumin was positively correlated with HIS (p = 0.025, r = 0.31), PHE and TRP (both p = 0.001, r = 0.46). HIS (p = 0.041), PHE (p = 0.047) and TRP (p = 0.044) concentrations were significantly lower in non-responders than in responders and partial responders.
CONCLUSIONS
CONCLUSIONS
This study may suggest further investigation on serum, HIS, PHE, TRP and TYR as markers of intestinal disease and proposed HIS, PHE and TRP as prognostic marker for response to therapy.
Identifiants
pubmed: 32321505
doi: 10.1186/s12917-020-02334-2
pii: 10.1186/s12917-020-02334-2
pmc: PMC7178940
doi:
Substances chimiques
Amino Acids
0
Biomarkers
0
Immunosuppressive Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
117Références
BMC Vet Res. 2016 Jun 16;12(1):114
pubmed: 27306031
J Comp Pathol. 2008 Feb-Apr;138 Suppl 1:S1-43
pubmed: 18336828
J Vet Intern Med. 2019 Jul;33(4):1602-1607
pubmed: 31111561
Br J Nutr. 2007 Aug;98(2):237-52
pubmed: 17403271
PLoS One. 2012;7(1):e31131
pubmed: 22303484
Tierarztl Prax Ausg K Kleintiere Heimtiere. 2018 Feb;46(1):15-20
pubmed: 29536459
J Vet Intern Med. 2010 Jan-Feb;24(1):10-26
pubmed: 20391635
World J Gastroenterol. 2017 May 28;23(20):3643-3654
pubmed: 28611517
World J Gastroenterol. 2014 Jan 7;20(1):163-74
pubmed: 24415869
J Small Anim Pract. 2016 Nov;57(11):589-599
pubmed: 27747868
PLoS One. 2015 Oct 16;10(10):e0140716
pubmed: 26474176
J Anim Sci. 2019 Jul 30;97(8):3274-3285
pubmed: 31363781
Mediators Inflamm. 2017;2017:6869259
pubmed: 28392631
Biomed Res Int. 2018 Feb 26;2018:9171905
pubmed: 29682569
World J Gastroenterol. 2016 Mar 21;22(11):3117-26
pubmed: 27003989
Mol Hum Reprod. 2015 May;21(5):389-409
pubmed: 25609213
J Vet Intern Med. 2007 Jul-Aug;21(4):700-8
pubmed: 17708389
J Nutr Biochem. 2010 Jun;21(6):468-75
pubmed: 19428234
J Pharmacol Exp Ther. 2016 Nov;359(2):238-246
pubmed: 27535977
Inflamm Bowel Dis. 2012 Jul;18(7):1214-20
pubmed: 21823214
PLoS One. 2019 Jun 10;14(6):e0218218
pubmed: 31181125
Int J Tryptophan Res. 2009 Mar 23;2:45-60
pubmed: 20651948
Mol Nutr Food Res. 2014 Jul;58(7):1474-90
pubmed: 24764203
J Vet Intern Med. 2018 May;32(3):1026-1032
pubmed: 29604114
PLoS One. 2011;6(7):e21230
pubmed: 21760889
Nutrients. 2017 Mar 21;9(3):
pubmed: 28335546
Vet J. 2015 Jul;205(1):28-32
pubmed: 26025135
Nutrients. 2018 Nov 16;10(11):
pubmed: 30453494
J Proteome Res. 2012 Jun 1;11(6):3344-57
pubmed: 22574726
Nutrients. 2017 Aug 23;9(9):
pubmed: 28832517