Development of a thermophilic coculture for corn fiber conversion to ethanol.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
22 04 2020
22 04 2020
Historique:
received:
14
06
2019
accepted:
25
03
2020
entrez:
24
4
2020
pubmed:
24
4
2020
medline:
1
8
2020
Statut:
epublish
Résumé
The fiber in corn kernels, currently unutilized in the corn to ethanol process, represents an opportunity for introduction of cellulose conversion technology. We report here that Clostridium thermocellum can solubilize over 90% of the carbohydrate in autoclaved corn fiber, including its hemicellulose component glucuronoarabinoxylan (GAX). However, Thermoanaerobacterium thermosaccharolyticum or several other described hemicellulose-fermenting thermophilic bacteria can only partially utilize this GAX. We describe the isolation of a previously undescribed organism, Herbinix spp. strain LL1355, from a thermophilic microbiome that can consume 85% of the recalcitrant GAX. We sequence its genome, and based on structural analysis of the GAX, identify six enzymes that hydrolyze GAX linkages. Combinations of up to four enzymes are successfully expressed in T. thermosaccharolyticum. Supplementation with these enzymes allows T. thermosaccharolyticum to consume 78% of the GAX compared to 53% by the parent strain and increases ethanol yield from corn fiber by 24%.
Identifiants
pubmed: 32321909
doi: 10.1038/s41467-020-15704-z
pii: 10.1038/s41467-020-15704-z
pmc: PMC7176698
doi:
Substances chimiques
Xylans
0
glucuronoarabinoxylan
0
Ethanol
3K9958V90M
Cellulose
9004-34-6
Types de publication
Evaluation Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1937Références
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