Enrichment and purification of red pigments from defective mulberry fruits using biotransformation in a liquid-liquid-solid three-phase system.
Biotransformation
Cyanidin-3-O-glucoside
Cyanidin-3-O-rutinoside
Immobilized enzyme
Liquid-liquid-solid three-phase system
Purification
Journal
Environmental science and pollution research international
ISSN: 1614-7499
Titre abrégé: Environ Sci Pollut Res Int
Pays: Germany
ID NLM: 9441769
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
received:
02
09
2019
accepted:
02
04
2020
pubmed:
24
4
2020
medline:
28
5
2021
entrez:
24
4
2020
Statut:
ppublish
Résumé
A large number of defective mulberries are discarded each year because mulberries are easy to break. The red pigments from defective mulberries are recognized as the sustainable sources of anthocyanins extracted from nature. Cyanidin-3-O-rutinoside and cyanidin-3-O-glucoside are the main components of mulberry red pigments, accounting for 50% and 40% of the total, respectively. Cyanidin-3-O-glucoside exhibits anticancer, hypoglycemic, and liver and visceral protection properties. Cyanidin-3-O-glucoside can be prepared by enzymatically hydrolyzing the rhamnosidase bond of cyanidin-3-O-rutinoside. To obtain mulberry red pigment with a high purity of cyanidin-3-O-glucoside, immobilized α-L-rhamnosidase was added to the aqueous two-phase system to construct a liquid-liquid-solid three-phase enzyme catalytic system. After optimization, the three-phase system was composed of 27.12% (w/w) ethanol, 18.10% (w/w) ammonium sulfate, 15% (w/w) mulberry juice, 4.24% (w/w) immobilized α-L-rhamnosidase, and 35.54% (w/w) pure water. The three-phase system was employed to enrich and purify cyanidin-3-O-glucoside at pH 5 and 45 °C for 1 h. The purity of cyanidin-3-O-glucoside was increased from 40 to 82.42% with cyanidin-3-O-rutinoside conversion of 60.68%. The immobilized α-L-rhamnosidase could be reused seven times, maintaining a relative activity of over 50%. Overall, the developed system provided an efficient and simple approach for high purity mulberry red pigment production and recycling in the field of sustainable agriculture. Graphical abstract.
Identifiants
pubmed: 32323230
doi: 10.1007/s11356-020-08731-2
pii: 10.1007/s11356-020-08731-2
doi:
Substances chimiques
Anthocyanins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
24432-24440Subventions
Organisme : Zhenjiang Science & Technology Program
ID : NY2017010
Organisme : 333 High-level Talent Training Project of Jiangsu Province
ID : BRA2019281
Organisme : Shen Lan Young scholars program of Jiangsu University of Science and Technology
ID : Year 2015
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