Expression and function of the nonclassical estrogen receptor, GPR30, in human cartilage and chondrocytes.
GPER
GPR30
cartilage
chondrocyte
estradiol
estrogen receptors
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
12
09
2019
revised:
24
03
2020
accepted:
25
03
2020
pubmed:
24
4
2020
medline:
17
3
2021
entrez:
24
4
2020
Statut:
ppublish
Résumé
Estrogen hormones are important for cartilage homeostasis, but nothing is known regarding the expression and role of the membrane G protein-coupled estrogen receptor (GPER), G protein-coupled receptor 30 (GPR30), in adult articular chondrocytes. Using immunohistochemistry of cartilage sections, quantitative real-time polymerase chain reaction and Western blot of chondrocyte extracts, we found that these cells express GPR30. Nonetheless, the pattern of bands detected by two distinct antibodies does not overlap, suggesting that the proteins detected represent partially degraded forms of the receptor. Treatment with GPR30 agonists did not induce Akt or ERK1/2 phosphorylation, two known GPR30-activated signaling pathways, suggesting that GPR30 is not functional in human chondrocytes. Therefore, the protective anti-osteoarthritic role of estrogen hormones in cartilage homeostasis is likely independent of GPR30. This study was performed using human cartilage collected from the distal femoral condyles of multiorgan donors at the Bone and Tissue Bank of the University and Hospital Center of Coimbra.
Substances chimiques
Estrogen Receptor alpha
0
Estrogens
0
GPER1 protein, human
0
Receptors, Estrogen
0
Receptors, G-Protein-Coupled
0
MAPK3 protein, human
EC 2.7.11.24
Mitogen-Activated Protein Kinase 3
EC 2.7.11.24
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8486-8494Informations de copyright
© 2020 Wiley Periodicals, Inc.
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