Non-chromatographic purification of thermostable endoglucanase from Thermotoga maritima by fusion with a hydrophobic elastin-like polypeptide.

Bacterial Proteins / biosynthesis Cellulase / biosynthesis Elastin / biosynthesis Escherichia coli / enzymology Recombinant Fusion Proteins / biosynthesis Thermotoga maritima / enzymology
Elastin-like polypeptide Endoglucanase Fusion expression Inverse transition cycling Thermostability

Journal

Protein expression and purification
ISSN: 1096-0279
Titre abrégé: Protein Expr Purif
Pays: United States
ID NLM: 9101496

Informations de publication

Date de publication:
09 2020
Historique:
received: 22 10 2019
revised: 26 02 2020
accepted: 31 03 2020
pubmed: 24 4 2020
medline: 22 1 2021
entrez: 24 4 2020
Statut: ppublish

Résumé

Endoglucanase EG12B from Thermotoga maritima is a thermophilic cellulase that has great potential for industrial applications. Here, to enable the selective purification of EG12B in a simple and efficient manner, an elastin-like polypeptide (ELP), which acts as a thermally responsive polypeptide, was fused with EG12B to enable its inverse phase transition cycling (ITC). A small gene library comprising ELPs from ELP

Identifiants

DOI: 10.1016/j.pep.2020.105634 PMID: 32325232
pubmed: 32325232
pii: S1046-5928(19)30567-4
doi: 10.1016/j.pep.2020.105634
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Recombinant Fusion Proteins 0
Elastin 9007-58-3
Cellulase EC 3.2.1.4

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

105634

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

Shanshan Wang (S)

School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, 723001, China; Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, 710069, China. Electronic address: jieyishanshan319@163.com.

Rui Lin (R)

Department of Gastroenterology and Hepatology, Tianjin Medical University, General Hospital, Tianjin, 300052, China.

Yanyan Ren (Y)

School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, 723001, China.

Tao Zhang (T)

School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, 723001, China.

Hongzhao Lu (H)

School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, 723001, China.

Ling Wang (L)

School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, 723001, China.

Daidi Fan (D)

Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, 710069, China. Electronic address: fandaidi@nwu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Non-chromatographic purification of...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Glucosidases › Cellulases › Cellulase › Non-chromatographic purification of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Scléroprotéines › Protéines de la matrice extracellulaire › Élastine › Non-chromatographic purification of...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Non-chromatographic purification of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Protéines de fusion recombinantes › Non-chromatographic purification of...
questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Bactéries anaérobies à Gram négatif › Bâtonnets à Gram négatif anaérobies droits, incurvés et spiralés › Thermotoga › Thermotoga maritima › Non-chromatographic purification of...