Increased blood lactate levels during exercise and mitochondrial DNA alterations converge on mitochondrial dysfunction in schizophrenia.


Journal

Schizophrenia research
ISSN: 1573-2509
Titre abrégé: Schizophr Res
Pays: Netherlands
ID NLM: 8804207

Informations de publication

Date de publication:
06 2020
Historique:
received: 23 07 2019
revised: 13 03 2020
accepted: 29 03 2020
pubmed: 25 4 2020
medline: 22 6 2021
entrez: 25 4 2020
Statut: ppublish

Résumé

Mitochondrial dysfunction and an elevation of lactate are observed in patients with schizophrenia (SZ). However, it is unknown whether mitochondrial dysfunction is associated with the presence of mitochondrial DNA (mtDNA) alterations and comorbid clinical conditions. We aimed to identify systemic mitochondrial abnormalities in blood samples of patients with SZ that may have a high impact on the brain due to its high bioenergetic requirements. Case/control study between 57 patients with SZ and 33 healthy controls (HCs). We measured lactate levels at baseline, during 15 min of exercise (at 5, 10 and 15 min) and at rest. We also evaluated the presence of clinical conditions associated with mitochondrial disorders (CAMDs), measured the neutrophil to lymphocyte ratio (NLR, a subclinical inflammatory marker), and analyzed mtDNA variation and copy number. Linear models adjusting for covariates showed that patients with SZ exhibited higher elevation of lactate than HCs during exercise but not at baseline or at rest. In accordance, patients showed higher number of CAMDs and lower mtDNA copy number. Interestingly, CAMDs correlated with both lactate levels and mtDNA copy number, which in turn correlated with the NLR. Finally, we identified 13 putative pathogenic variants in the mtDNA of 11 participants with SZ not present in HCs, together with a lactate elevation during exercise that was significantly higher in these 11 carriers than in the noncarriers. These results are consistent with systemic mitochondrial malfunctioning in SZ and pinpoint lactate metabolism and mtDNA as targets for potential therapeutic treatments.

Sections du résumé

BACKGROUND
Mitochondrial dysfunction and an elevation of lactate are observed in patients with schizophrenia (SZ). However, it is unknown whether mitochondrial dysfunction is associated with the presence of mitochondrial DNA (mtDNA) alterations and comorbid clinical conditions. We aimed to identify systemic mitochondrial abnormalities in blood samples of patients with SZ that may have a high impact on the brain due to its high bioenergetic requirements.
METHODS
Case/control study between 57 patients with SZ and 33 healthy controls (HCs). We measured lactate levels at baseline, during 15 min of exercise (at 5, 10 and 15 min) and at rest. We also evaluated the presence of clinical conditions associated with mitochondrial disorders (CAMDs), measured the neutrophil to lymphocyte ratio (NLR, a subclinical inflammatory marker), and analyzed mtDNA variation and copy number.
RESULTS
Linear models adjusting for covariates showed that patients with SZ exhibited higher elevation of lactate than HCs during exercise but not at baseline or at rest. In accordance, patients showed higher number of CAMDs and lower mtDNA copy number. Interestingly, CAMDs correlated with both lactate levels and mtDNA copy number, which in turn correlated with the NLR. Finally, we identified 13 putative pathogenic variants in the mtDNA of 11 participants with SZ not present in HCs, together with a lactate elevation during exercise that was significantly higher in these 11 carriers than in the noncarriers.
CONCLUSIONS
These results are consistent with systemic mitochondrial malfunctioning in SZ and pinpoint lactate metabolism and mtDNA as targets for potential therapeutic treatments.

Identifiants

pubmed: 32327316
pii: S0920-9964(20)30181-X
doi: 10.1016/j.schres.2020.03.070
pii:
doi:

Substances chimiques

DNA, Mitochondrial 0
Lactates 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

61-68

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors have no conflicts of interest to report, financial or otherwise.

Auteurs

Alba Valiente-Pallejà (A)

Research Department, Hospital Universitari Institut Pere Mata (HUIPM), Universitat Rovira I Virgili (URV), E43206 Reus, Catalonia, Spain; Institut d'Investigació Sanitària Pere Virgili (IISPV), E43204 Reus, Catalonia, Spain; Centro de Investigación Biomédica en Red en Salud Mental (CIBERSAM), E43204 Reus, Catalonia, Spain.

Helena Torrell (H)

Center for Omic Sciences (COS), Joint Unit Universitat Rovira i Virgili-EURECAT Technology Centre of Catalonia, Unique Scientific and Technical Infrastructures, Reus, Spain, 43204 Reus, Catalonia, Spain.

Yolanda Alonso (Y)

Research Department, Hospital Universitari Institut Pere Mata (HUIPM), Universitat Rovira I Virgili (URV), E43206 Reus, Catalonia, Spain; Institut d'Investigació Sanitària Pere Virgili (IISPV), E43204 Reus, Catalonia, Spain; Centro de Investigación Biomédica en Red en Salud Mental (CIBERSAM), E43204 Reus, Catalonia, Spain.

Elisabet Vilella (E)

Research Department, Hospital Universitari Institut Pere Mata (HUIPM), Universitat Rovira I Virgili (URV), E43206 Reus, Catalonia, Spain; Institut d'Investigació Sanitària Pere Virgili (IISPV), E43204 Reus, Catalonia, Spain; Centro de Investigación Biomédica en Red en Salud Mental (CIBERSAM), E43204 Reus, Catalonia, Spain.

Gerard Muntané (G)

Research Department, Hospital Universitari Institut Pere Mata (HUIPM), Universitat Rovira I Virgili (URV), E43206 Reus, Catalonia, Spain; Institut d'Investigació Sanitària Pere Virgili (IISPV), E43204 Reus, Catalonia, Spain; Centro de Investigación Biomédica en Red en Salud Mental (CIBERSAM), E43204 Reus, Catalonia, Spain; Institute of Evolutionary Biology (IBE), Spanish National Research Council (CSIC), Universitat Pompeu Fabra (UPF), E08003 Barcelona, Catalonia, Spain. Electronic address: muntaneg@peremata.com.

Lourdes Martorell (L)

Research Department, Hospital Universitari Institut Pere Mata (HUIPM), Universitat Rovira I Virgili (URV), E43206 Reus, Catalonia, Spain; Institut d'Investigació Sanitària Pere Virgili (IISPV), E43204 Reus, Catalonia, Spain; Centro de Investigación Biomédica en Red en Salud Mental (CIBERSAM), E43204 Reus, Catalonia, Spain. Electronic address: martorelll@peremata.com.

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Classifications MeSH