LPS Induces Active HMGB1 Release From Hepatocytes Into Exosomes Through the Coordinated Activities of TLR4 and Caspase-11/GSDMD Signaling.


Journal

Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960

Informations de publication

Date de publication:
2020
Historique:
received: 24 11 2019
accepted: 28 01 2020
entrez: 25 4 2020
pubmed: 25 4 2020
medline: 19 3 2021
Statut: epublish

Résumé

High-mobility group box-1 (HMGB1), a ubiquitous nuclear protein, acts as a late mediator of lethality when released extracellularly during sepsis. The major source of circulating HMGB1 in sepsis is hepatocytes. However, the mechanism of HMGB1 release of hepatocytes during sepsis is not very clear. We have previously shown that bacterial endotoxin [lipopolysaccharide (LPS)] sensing pathways, including Toll-like receptor (TLR)4 and caspase-11, regulate hepatocyte HMGB1 release in response to LPS. Here, we report the novel function of caspase-11 and gasdermin D (GsdmD) in LPS-induced active HMGB1 released from hepatocytes. HMGB1 release during endotoxemia was caspase-11/GsdmD dependent via an active way

Identifiants

pubmed: 32328059
doi: 10.3389/fimmu.2020.00229
pmc: PMC7160675
doi:

Substances chimiques

Gsdmd protein, mouse 0
HMGB1 Protein 0
Intracellular Signaling Peptides and Proteins 0
Lipopolysaccharides 0
Phosphate-Binding Proteins 0
Toll-Like Receptor 4 0
Calcium-Calmodulin-Dependent Protein Kinase Kinase EC 2.7.11.17
Casp4 protein, mouse EC 3.4.22.-
Caspases, Initiator EC 3.4.22.-
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

229

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM102146
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL141080
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM127027
Pays : United States

Informations de copyright

Copyright © 2020 Li, Deng, Loughran, Yang, Lin, Yang, Gao, Jin, Li, Cai, Lu, Billiar and Scott.

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Auteurs

Wenbo Li (W)

Department of Burn and Plastic Surgery, The Second Xiangya Hospital of Central South University, Changsha, China.
Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.

Meihong Deng (M)

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.

Patricia A Loughran (PA)

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.
Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA, United States.

Muqing Yang (M)

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.
Department of Surgery, Tenth People's Hospital of Tongji University, Shanghai, China.

Minjie Lin (M)

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.
The Second Xiangya Hospital of Central South University, Clinical Skills Training Center, Changsha, China.

Chenxuan Yang (C)

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.
School of Medicine, Tsinghua University, Beijing, China.

Wentao Gao (W)

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.

Shuqing Jin (S)

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.
Department of Anesthesiology, Shanghai East Hospital, Tongji University, Shanghai, China.

Shilai Li (S)

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.
Department of Emergency, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Jingjing Cai (J)

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.
Department of Cardiology, The Third Xiangya Hospital, Central South University, Changsha, China.

Ben Lu (B)

Department of Hematopathology, The Third Xiangya Hospital, Central South University, Changsha, China.

Timothy R Billiar (TR)

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.
Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA, United States.

Melanie J Scott (MJ)

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.
Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA, United States.

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Classifications MeSH