A Matter of Choice: Inhibition of c-Rel Shifts Neuronal to Oligodendroglial Fate in Human Stem Cells.
Adult
Adult Stem Cells
/ cytology
Animals
Biomarkers
/ metabolism
Cell Differentiation
/ drug effects
Cell Survival
/ drug effects
Cells, Cultured
Demyelinating Diseases
/ pathology
Glutamates
/ metabolism
Humans
Mice
Models, Biological
Myelin Sheath
/ metabolism
NF-kappa B
/ metabolism
Neural Crest
/ cytology
Neural Stem Cells
/ cytology
Neurons
/ cytology
Oligodendroglia
/ cytology
Pentoxifylline
/ pharmacology
Protein Subunits
/ metabolism
Proto-Oncogene Proteins c-rel
/ antagonists & inhibitors
Stem Cell Transplantation
MBP
NF-κB-c-REL
oligodendroglial fate shift
pentoxifylline
survival
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
22 04 2020
22 04 2020
Historique:
received:
16
03
2020
revised:
18
04
2020
accepted:
20
04
2020
entrez:
26
4
2020
pubmed:
26
4
2020
medline:
20
2
2021
Statut:
epublish
Résumé
The molecular mechanisms underlying fate decisions of human neural stem cells (hNSCs) between neurogenesis and gliogenesis are critical during neuronal development and neurodegenerative diseases. Despite its crucial role in the murine nervous system, the potential role of the transcription factor NF-κB in the neuronal development of hNSCs is poorly understood. Here, we analyzed NF-κB subunit distribution during glutamatergic differentiation of hNSCs originating from neural crest-derived stem cells. We observed several peaks of specific NF-κB subunits. The most prominent nuclear peak was shown by c-REL subunit during a period of 2-5 days after differentiation onset. Furthermore, c-REL inhibition with pentoxifylline (PTXF) resulted in a complete shift towards oligodendroglial fate, as demonstrated by the presence of OLIG2
Identifiants
pubmed: 32331232
pii: cells9041037
doi: 10.3390/cells9041037
pmc: PMC7226153
pii:
doi:
Substances chimiques
Biomarkers
0
Glutamates
0
NF-kappa B
0
Protein Subunits
0
Proto-Oncogene Proteins c-rel
0
Pentoxifylline
SD6QCT3TSU
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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