Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion.
Animals
Bacterial Adhesion
Cell Line
Disease Models, Animal
Epithelial Cells
Flagella
/ metabolism
Flagellin
/ metabolism
Histone-Lysine N-Methyltransferase
/ metabolism
Humans
Hydrophobic and Hydrophilic Interactions
Intestinal Mucosa
/ microbiology
Methylation
Mice
NIH 3T3 Cells
Protein Processing, Post-Translational
Salmonella Infections
/ microbiology
Salmonella typhimurium
/ metabolism
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
24 04 2020
24 04 2020
Historique:
received:
09
09
2019
accepted:
13
03
2020
entrez:
26
4
2020
pubmed:
26
4
2020
medline:
11
8
2020
Statut:
epublish
Résumé
The long external filament of bacterial flagella is composed of several thousand copies of a single protein, flagellin. Here, we explore the role played by lysine methylation of flagellin in Salmonella, which requires the methylase FliB. We show that both flagellins of Salmonella enterica serovar Typhimurium, FliC and FljB, are methylated at surface-exposed lysine residues by FliB. A Salmonella Typhimurium mutant deficient in flagellin methylation is outcompeted for gut colonization in a gastroenteritis mouse model, and methylation of flagellin promotes bacterial invasion of epithelial cells in vitro. Lysine methylation increases the surface hydrophobicity of flagellin, and enhances flagella-dependent adhesion of Salmonella to phosphatidylcholine vesicles and epithelial cells. Therefore, posttranslational methylation of flagellin facilitates adhesion of Salmonella Typhimurium to hydrophobic host cell surfaces, and contributes to efficient gut colonization and host infection.
Identifiants
pubmed: 32332720
doi: 10.1038/s41467-020-15738-3
pii: 10.1038/s41467-020-15738-3
pmc: PMC7181671
doi:
Substances chimiques
FljB protein, Salmonella
0
Flagellin
12777-81-0
Histone-Lysine N-Methyltransferase
EC 2.1.1.43
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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